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藏猪FABPs基因PCR-RFLP多样性研究
引用本文:刘志成,强巴央宗,张浩,辜雪冬,杨涛,吴常信.藏猪FABPs基因PCR-RFLP多样性研究[J].中国农业大学学报,2009,14(6).
作者姓名:刘志成  强巴央宗  张浩  辜雪冬  杨涛  吴常信
作者单位:1. 中国农业大学,动物科学技术学院,北京,100193
2. 西藏农牧学院,动物科学系,西藏,林芝,860000
基金项目:国家科技支撑项目(2008BADB2B01); 西藏自治区科技计划项目
摘    要:采用PCR-RFLP技术检测藏猪H-FABP和A-FABP基因遗传多态性。结果发现藏猪H-FABP-HinfⅠ酶切出现3种基因型,其中基因型HH频率最高,为63.39%;A-FABP-BsmⅠ酶切出现3种基因型,其中基因型AA频率最高,为55.36%;上述基因型分布均符合Hardy-Weinberg平衡。藏猪这2个基因均呈多态分布,与高IMF含量相关的基因型频率较高,这可能与藏猪优良的肉质有关系。

关 键 词:藏猪  FABPs基因  PCR-RFLP  多态性  

Research on PCR-RFLP polymorphisms of FABPs genes in Tibet pigs
LIU Zhi-cheng,CHAMBA Yangzom,ZHANG Hao,GU Xue-dong,YANG Tao,WU Chang-xin.Research on PCR-RFLP polymorphisms of FABPs genes in Tibet pigs[J].Journal of China Agricultural University,2009,14(6).
Authors:LIU Zhi-cheng  CHAMBA Yangzom  ZHANG Hao  GU Xue-dong  YANG Tao  WU Chang-xin
Institution:LIU Zhi-cheng1,CHAMBA Yangzom2,ZHANG Hao1,GU Xue-dong2,YANG Tao2,WU Chang-xin1(1.College of Animal Science , Technology,China Agricultural University,Bejing 100193,China,2.College of Agriculture , Animal Husb,ry,Tibet University,Linzhi 860000,China)
Abstract:Tibet pig is a special Chinese indigenous pig breed native to high-altitude areas of Tibet,with great ability of fat depositing,excellent meat quality and high intramuscle fat content. In the present study,the polymorphisms of A-FABP and A-FABP genes were discovered with the method of PCR-RFLP in Tibet pig. The results showed that there were three genotypes for H-FABP-PCR-Hinf I-RFLP,in which HH was the predominant genotype; and there are three genotypes for A-FABP-PCR-Bsm I-RFLP,in which AA was the predomi...
Keywords:Tibet pig  FABPs genes  PCR-RFLP  polymorphism  
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