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| 红将军苹果的脱毒与检测技术研究 | |
| 引用本文： | 苏佳明,段小娜,于强,徐维华,姜中武,董雅凤,陈学森.红将军苹果的脱毒与检测技术研究[J].华北农学报,2009,24(Z2). |
| 作者姓名： | 苏佳明 段小娜 于强 徐维华 姜中武 董雅凤 陈学森 |
| 作者单位： | 1. 山东省烟台市农业科学研究院,山东,烟台,265500;山东农业大学园艺科学与工程学院,山东,泰安,271018 2. 山东省烟台市农业科学研究院,山东,烟台,265500 3. 中国农业科学院果树研究所,辽宁,兴城,125100 4. 山东农业大学园艺科学与工程学院,山东,泰安,271018 |
| 基金项目： | 国家高技术研究发展计划(863计划)重点项目，山东省科技攻关计划项目 |
| 摘 要： | 以中熟红富士品种红将军的继代培养4～5代试管苗为试材,进行脱毒处理,并利用ELISA和RT-PCR技术检测脱毒效果.结果表明:红将军苹果试管苗在38℃高温条件下恒定热处理20 d,再二次茎尖培养后,试管苗脱毒率达86.4%～100%,可以有效脱除ASSVd、ASGV、ASPV、ApMV和ACLSV等主要的苹果病毒. |
| 关 键 词： | 苹果 脱毒 |
Virus Elimination and Detection Technique in Red General Fuji Apple Cultivar (Malus domestica) |
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| SU Jia-ming,DUAN Xiao-na,YU Qiang,XU Wei-hua,JIANG Zhong-wu,DONG Ya-feng,CHEN Xue-sen.Virus Elimination and Detection Technique in Red General Fuji Apple Cultivar (Malus domestica)[J].Acta Agriculturae Boreali-Sinica,2009,24(Z2). | |
| Authors: | SU Jia-ming DUAN Xiao-na YU Qiang XU Wei-hua JIANG Zhong-wu DONG Ya-feng CHEN Xue-sen |
| Abstract: | In vitro seedlings subeultured 4 -5 generation of midseason Fuji apple variety Red General were eliminated viruses,and then were detected by ELISA and RT-PCR. Results showed that keeping constant heat treatment conditions at 38℃ for 20 days,the virus elimination rate of Red General apple reached 86.4% -100%, and major apple viruses such as ASSVd,ASGV,ASPV,ApMV and ACLSV were eluted well. |
| Keywords: | ELISA RT-PCR Apple Virus elimination ELISA RT-PCR |
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