应用RAPD标记鉴定结球甘蓝品种

利用RAPD标记对17个结球甘蓝品种进行了分析。结果显示较低浓度基因组DNA、高浓度Taq酶及一致的Mg^2 浓度，有利于RAPD结果的稳定。所有品种经12个引物扩增后，发现利用其中4个引物在17个品种间所扩增出的多态性标记可将全部品种鉴别。17个品种的遗传多样性分析显示，秋冬甘蓝品种内的遗传差异较春甘蓝品种内更大。因此，为保持结球甘蓝多样性，将更多的遗传资源用于春甘蓝品种选育是必要的。

IDENTIFICATION OF CABBAGE CULTIVARS WITH RAPD MARKERS

Seventeen cabbage varieties were studied by means of RAPD markers in this research. It was shown that low concentration of genomic DNA, high concentration Tag DNA polymerase and similar Mg 2+ concentration in PCR reaction were beneficial for the stability of the results of RAPD. Twelve primers were used in DNA amplification, and 4 of them were found to show abundant polymorphism, sufficient to distinguish the 17 varieties. More intravarietal genetic diversity was observed in autumn-winter cabbage varieties than in spring varieties, suggesting that more germplasms should be used in cabbage breeding so as to maintain the diversity of the whole species of Brassica oleracea.