| 水稻单胚多芽突变体多芽发生的胚胎学基础 |
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| 引用本文： | 郭静玉,陈志雄,杨秉耀,陈新芬,刘向东,卢永根.水稻单胚多芽突变体多芽发生的胚胎学基础[J].作物学报,2011,37(12):2251-2260. |
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| 作者姓名： | 郭静玉 陈志雄 杨秉耀 陈新芬 刘向东 卢永根 |
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| 作者单位： | 1亚热带农业生物资源保护与利用国家重点实验室, 广东广州 510642; 2华南农业大学电镜室, 广东广州 510642 |
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| 基金项目： | 国家自然科学基金，国家公益性行业(农业)科研专项，广东省自然科学重点基金 |
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| 摘 要： | 植物胚胎发育过程中茎端分生组织的正常形成与发育是产生叶片、侧芽和茎的重要前提。水稻突变体4001的籽粒萌发时产生多个芽，为重要的茎端分生组织分化异常突变体。利用整体染色透明激光扫描共聚焦显微术(WE-CLSM)、塑料半薄切片术和扫描电子显微镜观察表明，突变体4001的胚囊结构和双受精过程正常，但胚胎发育和分化迟于野生型水稻，胚胎分化产生多个茎端分生组织，且位置异常。多个茎端分生组织的分化是4001多芽的胚胎学基础，与其相关的基因在调控水稻茎端分生组织分化的时间、未分化细胞数目以及背腹轴性建立等方面起着重要的作用。
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| 关 键 词： | 水稻 (Oryza sativa L.) 茎端分生组织 胚胎发育 |
| 收稿时间： | 2011-05-05 |
Differentiation of Multiple Shoot Apical Meristems in Mutant Rice with One Embryo Causing Multiple Plumuples |
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| GUO Jing-Yu,CHEN Zhi-Xiong,YANG Bing-Yao,CHEN Xin-Fen,LIU Xiang-Dong,LU Yong-Gen.Differentiation of Multiple Shoot Apical Meristems in Mutant Rice with One Embryo Causing Multiple Plumuples[J].Acta Agronomica Sinica,2011,37(12):2251-2260. |
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| Authors: | GUO Jing-Yu CHEN Zhi-Xiong YANG Bing-Yao CHEN Xin-Fen LIU Xiang-Dong LU Yong-Gen |
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| Institution: | 1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agriculture University, Guangzhou 510642, China;2.Electronic Microscope Laboratory, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | The proper formation and development of shoot apical meristem during plant embryonic development is prerequisite to generate leaves,buds and stems.Rice(Oryza sativa L.) mutant line 4001 produces multiple plumules in one seed and is consi dered as an important mutant with abnormal differentiation of shoot apical meristem.While the embryonic basis of the mutant with multiple plumules is yet unknown.To elucidate the cytological basis,mature embryo sac structure and process of double fertilization were observed... |
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| Keywords: | Rice(Oryza sativa L ) Shoot apical meristem Embryonic development |
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