| 红菇菌丝液体培养营养因子优化组合研究 |
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| 引用本文： | 张斌,周再知,陈羽,梁坤南,余雪标.红菇菌丝液体培养营养因子优化组合研究[J].安徽农业科学,2009,37(25):11871-11873. |
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| 作者姓名： | 张斌 周再知 陈羽 梁坤南 余雪标 |
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| 作者单位： | 中国林业科学研究院热带林业研究所,广东广州,510520;海南大学,海南儋州,571737;中国林业科学研究院热带林业研究所,广东广州,510520;海南大学,海南儋州,571737 |
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| 基金项目： | 国家‘十一五'林业科技支撑计划‘天然林非木材资源高效复合经营技术(2006BAD03A0405)专题'部分研究内容 |
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| 摘 要： | 目的]通过对常规培养基营养因子优化，筛选出适宜红菇菌丝体生长的最佳培养基配方，为红菇的仿生栽培和深层培养生产提供理论参考。方法]采用液体发酵培养法，研究葡萄糖、（NH4）2SO4、ZnSO4和VB1在不同质量浓度下对红菇纯培养菌株菌丝体生物量的影响，并用正交试验法筛选出适宜红菇菌丝体生长的最佳培养基配方。结果]结果表明，适宜红菇菌丝体生长的葡萄糖质量浓度为10．00-25．00g／L，（NH4）2SO4为2．07～4．83g/L，ZnSO4为4．50～6．50g／L，VB1为0．15—0．20g／L。红菇菌丝体生长的最佳培养基配方为20％马铃薯汁＋葡萄糖20．00g／L＋（NH4）2SO42．07g／L＋ZnSO45．50g／L＋VBB10．20g／L＋KH2PO43．00g／L。结论]用该培养基配方进行温室振荡发酵试验时，得到红菇最大菌丝产量为0．64g／L。
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| 关 键 词： | 红菇 营养因子 优化组合 |
Research on the Optimum Combination of Nutritional Factors of Liquid Culture of Russual sp. |
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| ZHANG Bin et al.Research on the Optimum Combination of Nutritional Factors of Liquid Culture of Russual sp.[J].Journal of Anhui Agricultural Sciences,2009,37(25):11871-11873. |
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| Authors: | ZHANG Bin |
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| Institution: | ZHANG Bin et al (Research Institute of Tropical Forestry,CAF,Guangzhou,Guangdong 510520) |
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| Abstract: | Objective] The aim was to screen the optimal formula of medium which was suitable for the growth of Russual sp.mycelium through optimizing the normal nutritional factors of medium,and provide theoretical reference for bionic cultivation and submerged culture production of Russual sp..Method] Using liquid fermentation and culture method,to research the effect of different quality concentrations of glucose,(NH4)2SO4,ZnSO4 and VB1 on pure culture mycelium biomass of Russual sp.,the optimal formula of medium ... |
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| Keywords: | Russual sp Nutritional factors Optimum combination |
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