| ISSR和RAPD分子标记技术在不同君子兰品种遗传多样性上的应用 |
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| 引用本文： | 郑玉红,钱美华,李莹,顾永华,彭峰.ISSR和RAPD分子标记技术在不同君子兰品种遗传多样性上的应用[J].北方园艺,2010(23):136-139. |
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| 作者姓名： | 郑玉红 钱美华 李莹 顾永华 彭峰 |
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| 作者单位： | 江苏省中科院植物研究所,南京中山植物园,南京,江苏,210014;江苏省中科院植物研究所,南京中山植物园,南京,江苏,210014;江苏省中科院植物研究所,南京中山植物园,南京,江苏,210014;江苏省中科院植物研究所,南京中山植物园,南京,江苏,210014;江苏省中科院植物研究所,南京中山植物园,南京,江苏,210014 |
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| 基金项目： | 江苏省农业科技成果转化资助项目 |
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| 摘 要： | 采用ISSR标记和RAPD标记技术研究了15个君子兰品种的遗传多样性。结果表明:从55条寡聚核苷酸引物中筛选出10条简单重复序列引物,共扩增出65条带,平均每条引物扩增出6.5条带,其中5.4条多态性带,多态性比率为81.95%;从55条寡聚核苷酸引物中筛选出11条多态性引物,共扩增出47条带,平均每条引物扩增出4.27条带,其中32条多态性带,多态性比率为67.88%。POPGEN软件计算出君子兰品种间遗传距离为0.1123~0.6330,平均值为0.3263。基于遗传相似系数的UPGMA聚类分析将15个品种明显聚为二组,国兰系列品种为一组,日本兰系列品种组成另一组。
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| 关 键 词： | 君子兰 品种 ISSR RAPD 遗传变异 |
Application on the Genetic Diversity of Different Species of Clivia miniata by ISSR and RAPD Molecular Markers |
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| ZHENG Yu-hong,QIAN Mei-hua,LI Ying,GU Yong-hua,PENG Feng.Application on the Genetic Diversity of Different Species of Clivia miniata by ISSR and RAPD Molecular Markers[J].Northern Horticulture,2010(23):136-139. |
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| Authors: | ZHENG Yu-hong QIAN Mei-hua LI Ying GU Yong-hua PENG Feng |
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| Institution: | ZHENG Yu-hong,QIAN Mei-hua,LI Ying,GU Yong-hua,PENG Feng (Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing Botanical Gardenm,Mem.Sun Yat-Sen,Nanjing,Jiangsu 210014) |
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| Abstract: | Genetic and variance on 15 cultivars of Clivia miniatawere studied by ISSR and RAPD marker.The results showed that the 65 loci were identified with 10 simple sequence repeat primers screened from 55 primers,out of which 54 were polymorphic and the percentage of polymorphic sites(PPS) was 81.95%;with each primers 6.5 bands were averagely amplified.47 loci were identified with 11 oligodeoxynucleotides primers screened from 55 primers,out of which 32 were polymorphic and the PPS was 67.88%;with each primers 4.... |
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| Keywords: | Clivia miniata cultivar ISSR RAPD genetic and variance |
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