| 柑桔溃疡病菌基因组DNA的微波法抽提及快速检测 |
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| 引用本文： | 姚廷山,周常勇,胡军华,冉春,李鸿筠,刘浩强,肖田.柑桔溃疡病菌基因组DNA的微波法抽提及快速检测[J].西南农业学报,2012,25(3):906-908. |
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| 作者姓名： | 姚廷山 周常勇 胡军华 冉春 李鸿筠 刘浩强 肖田 |
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| 作者单位： | 西南大学柑桔研究所,中国农业科学院柑桔研究所,重庆400712 |
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| 基金项目： | 农业部行业公益项目，中央高校基本科研业务费 |
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| 摘 要： | 利用微波热震惊提取固体表面柑桔溃疡病基因组DNA并加以优化,所得到的基因组DNA可作为PCR反应的模板进行16SrDNA基因有效扩增。与柑桔溃疡病基因组DNA其他抽提方法相比较,微波法更适用于该病的快速检测,具有快速、简便、费用低廉等特点,且对设备的要求不高,用特异性引物XCF/XCR可实现柑桔溃疡病菌的快速鉴定。
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| 关 键 词： | 柑桔溃疡病 微波 基因组DNA抽提 |
Microwave-based Method for Genomic DNA Extraction and Rapid Detection Method from Xanthomonas axonopodis pv.citri |
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| YAO Ting-shan
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ZHOU Chang-yong
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HU Jun-hua
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RAN Chun
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LI Hong-jun
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LIU Hao-qiang
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XIAO Tian.Microwave-based Method for Genomic DNA Extraction and Rapid Detection Method from Xanthomonas axonopodis pv.citri[J].Southwest China Journal of Agricultural Sciences,2012,25(3):906-908. |
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| Authors: | YAO Ting-shan ZHOU Chang-yong HU Jun-hua RAN Chun LI Hong-jun LIU Hao-qiang XIAO Tian |
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| Institution: | (Citrus Research Institute,Southwest University;Citrus Research Institute,Chinese Academy of Agricultrual Sciences,Chongqing 400712,China) |
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| Abstract: | The optimized microwave extraction was conducted to extract the genome of citrus canker disease.The genome extraction method was fast,easy to handle with low cost.The extracted DNA was suitable for PCR.The microwave-based method was suitable for fast identification compare to other methods.The method was effective,easy and fast.so it was recommended that the extraction method was applied for the detection of Xanthomonas axonopodis pv.citri using primers XCF/XCR. |
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| Keywords: | Citrus canker disease Microwave Genomic DNA extraction |
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