湖北小连翘与贯叶连翘中金丝桃素的含量比较

目的]比较测定贯叶连翘和湖北小连翘中金丝桃素的含量,以期为湖北小连翘这一新金丝桃素植物资源的开发利用奠定基础。方法]采用HPLC法测定金丝桃素含量,色谱条件为：Supelcosi lODS色谱柱（4.6mm×250mm,5μm）,流动相为甲醇-乙腈-水-0.3%磷酸,（V∶V=45∶50∶4.5∶0.5,用三乙胺调pH值至6.0）,流速为1ml/min,检测波长为590nm。结果]相同生长环境下的湖北小连翘中金丝桃素含量约为贯叶连翘中的3倍;湖北小连翘中金丝桃素的含量随着海拔高度的升高呈逐渐下降趋势;金丝桃素在湖北小连翘各部位中以花和幼果中含量最高,可达0.617%～0.877%,在叶和成熟果实中的含量约为0.35%,根和茎中含量极低。结论]该试验为湖北小连翘的人工种植和科学采收研究提供了理论指导,为湖北小连翘这一新金丝桃素植物资源的开发利用奠定基础。

Comparison of Hypericin Contents in Hypericum hubeiense and Hypericum perforatum

Objective]The contents of hypericin in Hypericum hubeiense and Hypericum perforatum were comparatively determined,aiming at providing basis for the development and utilization of Hypericum hubeiense.Method]HPLC was used,and the chromatographic conditions were as follows:Supelcosil ODS (4.6 mm×250 mm,5 μm) column,mobile phase of methanol-acetonitrile-water-0.3% aqueous solution of phosphoric acid (v∶v=45∶50∶4.5∶0.5,adjusting the pH to 6 with triethylamine) ,flow rate of 1 ml/min and detection wavelength of 590 nm.Result]The content of hypericin in Hypericum hubeiense was 3 times as that in Hypericum perforatum growing in the same environ-ment and decreased with the increasing elevation of its growth.Meanwhile,the hypericin content in the flowers and young fruits of Hypericum hubeiense was the highest which was 0.617%-0.877% ,and that in leaves and ripe fruits was about 0.35% ,while that in roots and stems was extremely low.Conclusion]This will provide theoretical basis for the artificial cultivation,scientific harvest and development of Hypericum hubeiense.