| 两种甜瓜病毒寿光分离物的分子检测与鉴定 |
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| 引用本文： | 乔宁,魏家鹏,李美芹,刘永光,姜会霞,竺晓平,田素波.两种甜瓜病毒寿光分离物的分子检测与鉴定[J].植物保护,2015,41(5):130-133. |
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| 作者姓名： | 乔宁 魏家鹏 李美芹 刘永光 姜会霞 竺晓平 田素波 |
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| 作者单位： | 1.潍坊科技学院，寿光262700; 2.山东农业大学植保学院，泰安271018; 3. 山东寿光蔬菜种业集团有限公司，寿光262700 |
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| 基金项目： | 公益性行业(农业)科研专项(201303028); 山东省自然科学基金(ZR2012CM032); 山东省科技发展计划(2010GNC10915); 潍坊市科技发展计划(201301159，2013YD177); 山东省农业良种工程现代种业企业科技培育计划 |
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| 摘 要： | 甜瓜坏死斑点病毒(Melon necrotic spot virus,MNSV)及瓜类褪绿黄化病毒(Cucurbit chlorotic yellows virus,CCYV)近年来在瓜类种植区均大面积发生,危害较为严重,已成为制约甜瓜生产的重要因素。本研究广泛收集疑似感染MNSV及CCYV的甜瓜病叶,从中提取植物总RNA进行RT-PCR扩增,将产物分别连接到pEASYT1Simple克隆载体上,对含有目的片段的重组子进行测序及比对分析。结果显示得到了与预期结果一致的DNA序列,其扩增产物大小分别为673bp(MNSV)和877bp(CCYV)。同源性分析结果表明,MNSV和CCYV寿光分离物的核苷酸序列与中国其他地区或一些国家已报道的分离物同源性达99%~100%。
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| 关 键 词： | 甜瓜坏死斑点病毒 瓜类褪绿黄化病毒 RT-PCR扩增 序列分析 |
| 收稿时间： | 2014/8/16 0:00:00 |
| 修稿时间： | 2014/10/26 0:00:00 |
Molecular detection and identification of two melon viruses from Shouguang |
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| Qiao Ning,Wei Jiapeng,Li Meiqin,Liu Yongguang,Jiang Huixi,Zhu Xiaoping,Tian Subo.Molecular detection and identification of two melon viruses from Shouguang[J].Plant Protection,2015,41(5):130-133. |
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| Authors: | Qiao Ning Wei Jiapeng Li Meiqin Liu Yongguang Jiang Huixi Zhu Xiaoping Tian Subo |
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| Institution: | 1. Weifang University of Science and Technology, Shouguang 262700, China; 2. College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China; 3. Shandong Shouguang Vegetable Seed Industrial Group, Shouguang 262700, China |
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| Abstract: | Melon necrotic spot virus (MNSV) and Cucurbit chlorotic yellows virus (CCYV) have seriously affected the yield and quality of melons in large melon growing areas in recent years. In this study, diseased leaves infected by MNSV and CCYV were widely collected from Shouguang, Shandong Province. Total RNA were extracted from these leaf tissues and RT-PCR was performed. The PCR products were inserted into pEASY T1 Simple cloning vector, and the expected DNA fragments with the size of 673 bp (MNSV) and 877 bp (CCYV) were sequenced and analyzed by BLAST search in GenBank, respectively. Homology analysis showed that nucleotide sequence of MNSV and CCYV isolates from Shouguang had 99%-100% identity with those isolates from other parts of China or some countries previously reported. |
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| Keywords: | MNSV CCYV RT-PCR sequence analysis |
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