| 3个烟草品种愈伤组织诱导的比较 |
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| 引用本文： | 钟林森.3个烟草品种愈伤组织诱导的比较[J].安徽农业科学,2011,39(27):16494-16495. |
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| 作者姓名： | 钟林森 |
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| 作者单位： | 湖南省龙山县烟草专卖局,湖南龙山,416800 |
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| 摘 要： | 目的]研究不同品种烟草在不同培养基上愈伤组织的诱导情况。方法]以烟草HY-9-7、云烟97和K326的幼嫩叶片为材料,分别在MS＋2,4-D 2.0 mg/L＋KT 0.2 mg/L和MS＋NAA 2.0 mg/L＋6-BA 2.0 mg/L培养基上诱导愈伤组织,选出愈伤组织诱导率最高的烟草品种和最优培养基。结果]MS＋2,4-D 2.0 mg/L＋KT 0.2 mg/L是诱导烟草愈伤组织的理想培养基;K326在最优培养基上诱导率最高,愈伤组织生长状态最好。结论]为烟草基因工程的研究和种质资源的创新提供理论支撑。
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| 关 键 词： | 烟草 愈伤组织 培养基 诱导率 |
Comparison of Callus Induction of 3 Tobacco Varieties |
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| ZHONG Lin-sen.Comparison of Callus Induction of 3 Tobacco Varieties[J].Journal of Anhui Agricultural Sciences,2011,39(27):16494-16495. |
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| Authors: | ZHONG Lin-sen |
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| Institution: | ZHONG Lin-sen(Tobacco company of Longshan County of Hunan Province,Longshan,Hunan 416800) |
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| Abstract: | Objective] The research aimed to study the callus induction of different tobacco varieties in different media.Method] With tender leaves of HY-9-7,Yunyan 97 and K326 as materials,callus was induced in the medium of MS+2,4-D 2.0 mg/L +KT 0.2 mg/L and MS+NAA 2.0 mg/L +6-BA 2.0 mg/L respectively,and then the tobacco variety of the highest callus induction rate and the optimal medium were selected.Result]The results showed that the optimum medium for callus induction was MS+2,4-D 2.0 mg/L +KT 0.2 mg/L;K326 h... |
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| Keywords: | Tobacco Callus Medium Induction rate |
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