| 白子菜快速繁殖研究 |
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| 引用本文： | 冯李,张发春. 白子菜快速繁殖研究[J]. 安徽农业科学, 2011, 39(27): 16489+16597-16489,16597 |
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| 作者姓名： | 冯李 张发春 |
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| 作者单位： | 重庆师范大学初等教育学院,重庆,400700;重庆师范大学初等教育学院,重庆,400700 |
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| 摘 要： | [目的]探究白子菜组织培养最佳培养基配方实现其快速繁殖。[方法]研究不同培养基配方对白子菜组织培养过程中愈伤组织的产生、芽和根分化效果的影响。[结果]④MS＋BA 1.5 mg/L＋NAA 1.2 mg/L＋蔗糖30 g/L＋琼脂粉6 g/L为白子菜形成愈伤组织的最佳培养基;⑤花宝一号＋BA 0.1 mg/L＋NAA 0.4 mg/L＋蔗糖30 g/L＋琼脂粉6 g/L为诱导愈伤组织形成芽的最佳培养基;瑏瑢MS＋BA 1.5 mg/L＋NAA 1.2 mg/L＋蔗糖20 g/L＋活性炭1 g/L＋琼脂粉6 g/L为最佳生根培养基。[结论]活性炭对白子菜组织培养有影响,具有抑制愈伤组织形成和促进生根的作用。
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| 关 键 词： | 白子菜 组织培养 诱导 |
Study on Tissue Culture of Gynura divaricata(Linn.) DC. |
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| FENG Li et al. Study on Tissue Culture of Gynura divaricata(Linn.) DC.[J]. Journal of Anhui Agricultural Sciences, 2011, 39(27): 16489+16597-16489,16597 |
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| Authors: | FENG Li et al |
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| Affiliation: | FENG Li et al(College of Elementary education,Chongqing Normal University,Chongqing 400700) |
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| Abstract: | [Objective] The aim was to explore the best culture medium formula of tissue culture of Gynura divaricata(Linn.) DC..[Method] The effects of different culture medium formula on callus initiation,differentiation of bud and root were studied.[Results] MS+BA 1.5 mg/L+NAA 1.2 mg/L+saccharose 30 g/L+gelose 6 g/L was the best culture medium of callus initiation of Gynura divaricata;HuaBao No.1+BA 0.1 mg/L+NAA 0.4 mg/L+saccharose 30 g/L gelose 6 g/Lwas the best culture medium which made callus form bud;MS+BA 1.5 m... |
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| Keywords: | Gynura divaricata(Linn.) DC. Tissue Culture Induction |
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