均匀设计法提取附子总生物碱的工艺研究

目的]筛选附子中总生物碱的最佳提取工艺寻找适合工业化生产的操作程序。方法]采用均匀设计法对中药附子中总生物碱进行提取,以样品总生物碱量为评价指标探讨乙醇提取的浓度、用量和提取时间对总提取量的影响,将优选出的最佳提取工艺条件,与其他文献中所使用的方法进行比较,判断是否适合工业化生产。即用TS-NS系列提取浓缩机组对附子进行提取浓缩,冷冻干燥后溶解并用滴定法测定测定附子中总生物碱的含量。结果]最佳工艺条件为：提取剂乙醇浓度为85%,附子粒径60目,乙醇用量为药材量的9倍（1∶9,g∶m l）,提取时间为2 h。在此条件下,总生物碱得率为0.312%。结论]该方法提取效果较好,可用于工业化生产。

Study on the Extraction Technology of Total Alkaloids from RADIX ACONITI LATERALIS PREPARATA by the Uniform Design Method

Objective] To screen the optimal extraction technology of total alkaloids from RADIX ACONITI LATERALIS PREPARATA,so as to find out the technology applicable to the industrial production.Method] The total alkaloids was extracted from RADIX ACONITI LATERALIS PREPARATA by the uniform design method,and then the effects of ethanol concentration,dose and extracting time on the total extracting yield were discussed,the optimal extraction technology was screened and then compared with the methods used in other li...