| 饲料中沙门氏菌的分离鉴定与Dot-ELISA检测 |
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| 引用本文： | 韩志辉,张红见.饲料中沙门氏菌的分离鉴定与Dot-ELISA检测[J].安徽农业科学,2011,39(26):16139-16140,16163. |
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| 作者姓名： | 韩志辉 张红见 |
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| 作者单位： | 青海大学农牧学院动物医学系,青海西宁,810016 |
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| 摘 要： | 目的]建立检测饲料中沙门氏菌的Dot-ELISA法。方法]采用Dot-ELISA法,对随机采集自西宁市某饲料厂的100份成品饲料、85份鱼粉和50份精蛋白进行了沙门氏菌检测。通过生化试验和血清学试验对饲料样品中的细菌进行了进一步的鉴定。结果]通过Dot-ELISA检测,成品饲料、鱼粉和精蛋白中的沙门氏菌阳性率分别为38.00%、62.35%和60.00%。Dot-ELISA法与常规分离鉴定技术的符合率为82.23%。结论]Dot-ELISA法具有较好的特异性和敏感性,与常规分离技术具有较高的符合率,可以用于饲料中沙门氏菌的检测。
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| 关 键 词： | Dot-ELISA 沙门氏菌 检测 饲料 |
Detection Salmonella in Forage by Dot-ELISA |
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| HAN Zhi-hui et al.Detection Salmonella in Forage by Dot-ELISA[J].Journal of Anhui Agricultural Sciences,2011,39(26):16139-16140,16163. |
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| Authors: | HAN Zhi-hui |
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| Institution: | HAN Zhi-hui et al(Department of Animal Medicine.Qinghai University,Xining,Qinghai 810016) |
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| Abstract: | Objective] To develop a Dot-ELISA(Dot-enzyme linked immunosorbent assay) method for detection of Salmonella in forage.Method] Forage samples including 100 finished feed samples,85 fishmeal samples and 50 protamine samples were randomly collected from a feed factory in Xining City,and Salmonella in these samples were detected by Dot-ELISA.The Salmonella isolates were further identified using biochemical tests and serological tests.Result] As detected by Dot-ELISA,the positive rates of Salmonella in the fi... |
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| Keywords: | Dot-enzyme linked immunosorbent assay Salmonella Detection Forage |
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