鸡腿蘑液体菌种生产及栽培试验研究

用深层发酵法进行鸡腿蘑液体菌种的生产，从5个鸡腿蘑品种中，确定了一株适于深层发酵的菌株一瑞10，该菌株较适宜的液体培养基组分为：4%玉米粉，2%蔗糖，2%麸皮，0.1%KH2PO4，0.05%MgSO4.7H2O，适宜的摇瓶条件为：培养基的起始pH值为7，250mL摇瓶装液量为40mL，接种量为10%，培养温度25℃.15L发酵罐控制参数为：装料系数0.7，接种量10%，通气量：1：0.5-1.2，搅拌速度为120-180r.min^-1，用液体菌种进行鸡腿蘑栽培试验结果显示，深层发酵法生产鸡腿蘑比传统栽培方式生产周期可缩短60d，抗杂性能优于传统生产方式，出菇性能与传统生产式无显著差异。

Studies on the liquid seed cultu re and cultivation of Coprinus coma tus

A strain suitable for subm eraged fermentation was selected from fi ve strains of Coprinus comatus．The st rai n’s suitable composition of fermentatio n medium was as follows：4％ maize powder ，2％ sugar，2％ wheat bran，0．1％ KH2PO 4，0．05％ MgSO4*7H2O．The optimal shaking culture conditions were as follows：pH7，4 0 mL medium in size 250mL flask，inoculat ion 10％，temperature 25℃．Fermentation pro cess controls were as follows：operationa l volume factor 0．7，inoculation 10％，gas through-puts 1∶0.5～1．2，mixing rate 120～18 0 r*mi n-1．The Coprinus comatus cultivation wi th liguid inoculum showed the production period was reduced by 60 days and the r esistance to contamination was better th an that produced in the traditional way， and thre wasn’t significant difference i n the productivity between the two ways.