| 茶树P5CS基因克隆及其在渗透和高盐胁迫下的表达分析 |
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| 引用本文： | 王丽珊,林清凡,陈兰平,池福铃,郭春芳,张积森.茶树P5CS基因克隆及其在渗透和高盐胁迫下的表达分析[J].热带作物学报,2015,36(1):68-75. |
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| 作者姓名： | 王丽珊 林清凡 陈兰平 池福铃 郭春芳 张积森 |
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| 作者单位： | 1. 福建师范大学生命科学学院,福建福州 350108 ;农业部甘蔗遗传改良重点开放实验室,福建福州 350108 ;福建农林大学基因组与生物技术研究中心,福建福州 350002
2. 福建教育学院,福建福州,350025 |
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| 基金项目： | 福建省自然科学基金项目（No. 2011J01091）；福建省教育厅科技项目（No. JA10279）。 |
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| 摘 要： | △1-吡咯琳-5-羧酸合成酶是植物脯氨酸合成过程中的关键酶。应用同源克隆方法获得茶树P5CS,序列长为1 316 bp,编码323个氨基酸;其编码蛋白分子量为34.7 ku,p I为7.62;N端有1个氨基酸激酶超家族Amino Acid Kinases(AAK)superfamily]功能区,C端有1个醛脱氢酶超家族Aldehyde Dehydrogenas(ALDH)superfamily]功能区,预测为亲水性跨膜蛋白;对18个物种的P5CS进行聚类分析,结果生物学分类及进化关系吻合。并与美味猕猴桃高度同源,相似度达89%。应用实时荧光定量PCR分析表明,该基因转录本在水分胁迫24 h内升至对照组水平的2.6倍,而高盐胁迫48 h后才升至9.9倍的最高值;水分胁迫应答速度快,但相对表达量较高盐胁迫低。由此推测该基因被诱导参与了渗透胁迫应答响应,并且对渗透胁迫中的旱害脱水更为敏感。
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| 关 键 词： | 茶树 P5CS 实时荧光定量PCR 水分胁迫 高盐胁迫 |
Cloning and Expression Analysis of P5CS Gene under Drought and Salinity Stress in Camellia sinensis |
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| WANG Lishan,LIN Qingfan,CHEN Lanping,CHI Fuling,GUO Chunfang and ZHANG Jisen.Cloning and Expression Analysis of P5CS Gene under Drought and Salinity Stress in Camellia sinensis[J].Chinese Journal of Tropical Crops,2015,36(1):68-75. |
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| Authors: | WANG Lishan LIN Qingfan CHEN Lanping CHI Fuling GUO Chunfang and ZHANG Jisen |
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| Institution: | 1 College of Life Sciences, Fujian Normal University 2 Key Lab for Sugarcane Genetic Improvement, Ministry of Agricultur 3 Genomics and Biotechnology Research Center, Fujian Agriculture and Forestry University;1 College of Life Sciences, Fujian Normal University 2 Key Lab for Sugarcane Genetic Improvement, Ministry of Agricultur 3 Genomics and Biotechnology Research Center, Fujian Agriculture and Forestry University;1 College of Life Sciences, Fujian Normal University 2 Key Lab for Sugarcane Genetic Improvement, Ministry of Agricultur 3 Genomics and Biotechnology Research Center, Fujian Agriculture and Forestry University;1 College of Life Sciences, Fujian Normal University 2 Key Lab for Sugarcane Genetic Improvement, Ministry of Agricultur 3 Genomics and Biotechnology Research Center, Fujian Agriculture and Forestry University;Fujian Institute of Education;1 College of Life Sciences, Fujian Normal University 2 Key Lab for Sugarcane Genetic Improvement, Ministry of Agricultur 3 Genomics and Biotechnology Research Center, Fujian Agriculture and Forestry University |
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| Abstract: | |
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| Keywords: | Camellia sinensis P5CS Real-time quantitative PCR Drought stress Salinity stress |
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