根癌农杆菌介导转Bt基因苎麻的获得及其抗虫鉴定

采用本研究室建立的根癌农杆菌介导的高效遗传转化体系，转化苎麻优良品种芦竹青，获得了转Bt基因苎麻候选植株。通过PCR和Southern杂交等分子检测，证明Bt基因已经整合到部分候选植株基因组中。选取PCR和Southern杂交均为阳性的部分株系(T₀)种植在大田，对这些植株进行室内抗虫鉴定并考察其主要农艺性状和品质性状，次年对T₁代植株进行PCR和Southern杂交检测。结果表明, 与对照相比，T₀代转Bt基因苎麻植株的抗虫性均强于对照，部分株系的抗虫性显著强于对照植株；且基本保持了亲本的优良性状；T₁代植株中也含有Bt基因，表明Bt基因能稳定遗传，且T₁代植株在大田的抗虫性明显强于非转基因植株。

Transgenic Ramie with Bt Gene Mediated by Agrobacterium tumefacien and Evaluation of Its Pest-Resistance

Agrobacterium tumefaciens-mediated approach is an effectively and widely used to introduce foreign DNA into plants. However, there were few reports published on ramie transformation mediated by Agrobacterium tumefaciens up to date. In the present study, transgenic ramie containing Bt gene were obtained by our highly efficient Agrobacterium-mediated transformation system using ramie Luzhuqing. Three T₀ plants of transgenic ramie were chosen for further experiments and were transplanted into the fields. Laboratory assay of artificial infestation and the survey on agronomic traits and quality traits of T₀ plants were conducted. The results showed that T₀ transgenic plants exhibited more excellent pest resistance than the controls, and some were significantly superior to the controls. In addition, T₀ transgenic plants maintained the main agronomic traits, such as plant height, stem diameter, rate of effective tiller, raw fiber weight, fiber production ratio from stem and fiber production ratio from phloem, and quality traits, such as strength, filament breaking tenacity, fiber fineness, fineness uniformity and glue content of raw fiber in Luzhuqing. PCR and Southern blotting analysis showed that T₁ plants contained Bt gene, which indicated that Bt gene could be stably inherited. T₁ plants in the fields performed highly pest resistance compared with non-transgenic plants.