菜心花粉萌发研究初报

目的]为菜心的通过雄性不育系的遗传育种提供参考。方法]以种植于花钵中的菜心为材料,采用TTC法测定其花粉活力;同时将花粉分别在3种培养基上培养,3种培养基即A1:15.00%蔗糖+0.01%硼酸+0.001%GA3,A2:15.00%蔗糖+0.40 mmol/L CaCl2+0.40 mmol/LH3BO3+1.00%琼脂,A3:5.00 mmol/LMES+1.00 mmol/L KCl+10.00 mmol/L CaCl2+0.80 mmol/LMgSO4+1.50 mmol/L硼酸+1.00%琼脂+16.60%蔗糖+3.65%山梨醇+10.00μg/ml肌醇,比较培养6 h和12 h后的花粉萌发率和花粉管长度。结果]试验表明,菜心花粉活力很高,具有活力的花粉比例平均达98.18%;A1培养基培养效果最好,A2次之,A3最差;培养12 h的花粉萌发率高于培养6 h的,但培养12 h的花粉管长度却小于培养6 h后的。结论]综合分析认为,菜心花粉在A1培养基即15.00%蔗糖+0.01%硼酸+0.001%GA3上培养6 h萌发效果最好。

Preliminary Repory on the Experiment in Pollen Germination of Mock Pad-choi

Objective] The trial was to provide the reference for mock pad-choi breeding through the male sterile line.Method] The pollen vitality including the germination rate and the length of pollen tube of mock pad-choi planted in vegetable bowl was determined with TTC determination at the same time.The plants were culture in three kinds of culture media,and three kinds of media were A1: 15.00% sucrose+0.01% boric acid+0.001% GA3;A2: 15.00% sucrose+0.40 mmol/L CaCl2 +0.40 mmol/L H3BO3+1.00% agar and A3: 5.00 mmol/L MES+1.00 mmol/L KCl+10.00 mmol/L CaCl2+0.80 mmol/L MgSO4+1.50 mmol/L boric acid agar+1.00%+3.65%+16.60% sucrose sorbitol+10.00 μg/ml inositol,and the testing result at 6h and 12 h was compared.Results] The result showed that mock pad-choi had high pollen vitality(average 98.18%).A1 culture medium was best;A2,second and A3 was worst.The pollen germination rate of 12 h growth was higher than that of the 6 h,but the tube length of 12 h growth was less than that of 6 h.Conclusion] The comprehensive analysis indicated that the pollen of mock pad-choi culture in A1 medium with 15.00 percent sucrose+0.01% boric acid+0.001% GA3 for 6 h was with best germination.