| 银莓离体快繁技术研究 |
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| 引用本文： | 李青梅,秦彩云,孙景花,侯星烨,陈士刚,陶晶.银莓离体快繁技术研究[J].吉林林业科技,2011,40(1):14-16,36. |
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| 作者姓名： | 李青梅 秦彩云 孙景花 侯星烨 陈士刚 陶晶 |
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| 作者单位： | 1. 吉林省林业科学研究院,吉林,长春,130033
2. 吉林市松花湖风景区园林管理处,吉林,吉林,132013 |
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| 基金项目： | 吉林省科技发展计划资助项目(20070216) |
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| 摘 要： | 以银莓当年生枝的顶芽和腋芽为外植体,以MS为基本培养基,添加不同配比的植物生长调节剂进行离体培养.结果表明:芽诱导最适宜的培养基为MS+6-BA3.0 mg·L-1+NAA3.0 mg·L-1+蔗糖30 g·L-1+琼脂4.5 g·L-1,增殖培养基为MS+6-BA1.0 mg·L-1+NAA1.0 mg·L-1+蔗糖...
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| 关 键 词： | 银莓 组织培养 快繁 |
Study on rapid propagation of Elaeagnus commutata |
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| LI Qing-mei,QIN Cai-yun,SUN Jing-hua,HOU Xing-ye,CHEN Shi-gang,TAO Jing.Study on rapid propagation of Elaeagnus commutata[J].Jilin Forestry Scicnce and Technology,2011,40(1):14-16,36. |
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| Authors: | LI Qing-mei QIN Cai-yun SUN Jing-hua HOU Xing-ye CHEN Shi-gang TAO Jing |
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| Institution: | LI Qing-mei1,QIN Cai-yun1,SUN Jing-hua1,HOU Xing-ye2,CHEN Shi-gang1,TAO Jing1(1.Jilin Provincial Academy of Forestry Sciences,Changchun 130033,China,2.Garden Administration Office of Sohua Lake in Jilin City,Jilin 132013) |
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| Abstract: | We used the terminal bud and axillary bud of Elaeagnus commutata in current year as the explant,and MS as basic culture medium with different combinations of plant growth regulators.The result showed that the best culture medium for bud induction was MS ＋ 6-BA3.0 mg ·L-1＋ NAA3.0 mg ·L-1＋ saccharose30 g·L-1＋ agar4.5 g·L-1,for propagation was MS ＋ 6-BA1.0 mg·L-1＋ NAA1.0 mg·L-1＋ saccharose30 g·L-1 ＋ agar4.5 g·L-1 and for rooting was 1/2MS ＋ NAA1.0 mg·L-1 ＋ saccharose20 g·L-1 ＋ agar4.5 g·L-1.The survival rate of transplantation was 85% if a mixture of rotten leaf soil： furnace ash（21） was used as soil substrate. |
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| Keywords: | Elaeagnus commutata tissue culture rapid propagation |
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