UPLC-MS/MS法检测稻米中苄嘧磺隆和苯噻酰草胺的残留量

建立了水稻中提取苄嘧磺隆和苯噻酰草胺残留量的液相色谱-质谱/质谱测定方法 UPLC-MS/MS。稻米样品经乙腈匀浆,上清液经氮气吹干浓缩,过PSA固相萃取柱净化,经UPLC-MS/MS测定,外标法定量。苄嘧磺隆在稻米中的最低检出量为0.1 ng,最低检出浓度为0.02 mg/kg,添加回收率为77.7%-91.5%,稻米的RSD为2.9%-8.5%;苯噻酰草胺在稻米中的最低检出量为0.05 ng,最低检出浓度为0.01 mg/kg,添加回收率为94.0%-109.0%,稻米的RSD为5.0%-8.2%。建立方法准确、快速、灵敏度高,能够满足苄嘧磺隆和苯噻酰草胺残留量分析的要求。

Determination of Bensulfuron-methyl and Mefenacet Residues in Rice by UPLC-MS/MS

Established the detection of bensulfuron-methyl and mefenacet residues in rice by UPLC-MS/MS methods. Rice samples were homogenized in acetonitrile, the supernatant was dried by nitrogen concentration, over PSA solid phase extraction （SPE）, measured by UPLC-MS/MS, external standard. Bensulfuron-methyl minimum detectable amount of rice is 0.1ng, the minimum detectable concentration of 0.02 mg/kg, recoveries were 77.7%-91.5%, rice RSD 2.9% to 8.5%; mefenacet in rice in the minimum detectable amount of 0.05 ng, the minimum detectable concentration of 0.01 mg/kg, recoveries were 94.0%-109.0%, rice RSD 5.0% to 8.2%. The method is accurate, rapid, sensitive and able to meet bensulfuron-methyl and mefenacet residue analysis.