| 用ITS序列识别牧草种质资源 |
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| 引用本文： | 姚娜,谭嘉力,赖志强,韦锦益,徐海雄. 用ITS序列识别牧草种质资源[J]. 广西农业生物科学, 2014, 0(1): 141-144 |
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| 作者姓名： | 姚娜 谭嘉力 赖志强 韦锦益 徐海雄 |
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| 作者单位： | [1]广西壮族自治区畜牧研究所,南宁530001 [2]广东产品质量监督检验研究院,国家食品质量监督检验中心（广东）,顺德528300 |
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| 基金项目： | 广西水产畜牧兽医局科技计划项目（桂渔牧科1304511）资助致谢 感谢广西壮族自治区畜牧研究所牧草研究室易显凤、梁永良、丘金花、邓素嫒和赖大伟等同志对试验的支持和帮助;感谢广东产品质量监督检验研究院和国家食品质量监督检验中心（广东）谭嘉力博士的技术指导. |
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| 摘 要： | DNA条形码技术能够快速、准确地识别物种,对于开展基础性的分类学研究和应用性的生物多样性研究极为重要。本文以广西畜牧研究所牧草种质资源圃的8个物种共22个品种为材料,进行植物条形码ITS测序。PCR结果显示ITS的扩增成功率达到100%,同源性的分析表明各物种中的ITS序列变异显著,共找到99个可以用来区分物种的变异位点,系统进化树的结果表明利用ITS序列进行物种水平鉴定成功率达100%,能够作为DNA条形码识别植物物种。
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| 关 键 词： | DNA条形码 核糖体内转录间隔区（ITS） 物种鉴别 |
Identification of Forage Germplasm Resources in ITS Sequence |
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| Yao Na,Tan Jiali,Lai Zhiqiang,Wei Jinyi,Xu Haixiong. Identification of Forage Germplasm Resources in ITS Sequence[J]. Journal of Guangxi Agricultural and Biological Science, 2014, 0(1): 141-144 |
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| Authors: | Yao Na Tan Jiali Lai Zhiqiang Wei Jinyi Xu Haixiong |
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| Affiliation: | 1 Guangxi Institute of Animal Science, Nanning, 530001; 2 China National Quality Supervision and Testing Center for Food (Guangdong), Guangdong Testing Institute of Product Quality Supervision, Shunde, 528300) |
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| Abstract: | It is crucial for the studies of taxonomy and biodiversity by using DNA barcode technology to fast and accurately make species identification. In this study, the 22 varieties from 8 plant species in Guangxi Institute of Animal Science were sampled and sequenced. Plant samples were processed with a classical DNA barcoding approach by amplifying and sequencing for ITS, and obtained 99 SNP to species identification. The SNP and neighbour-joining tree were employed to test the reliability ofbarcode at the species level. The results showed that ITS had significant divergence and high species identification reliability （100%）, and it was recommended as a test fragment ofbarcoding to species identification. |
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| Keywords: | DNA barcodes Internal transcribed spacers （ITS） Species identification |
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