不同浓度激素对省沽油组培过程中芽增殖影响的研究 |
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引用本文: | 陶许一,邵庆,毕璋友.不同浓度激素对省沽油组培过程中芽增殖影响的研究[J].安徽农业科学,2013(29):11718-11719. |
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作者姓名: | 陶许一 邵庆 毕璋友 |
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作者单位: | 安庆职业技术学院,安徽安庆,246003;安庆职业技术学院,安徽安庆,246003;安庆职业技术学院,安徽安庆,246003 |
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基金项目: | 安徽省高校自然科学研究课题(KJ20108099). |
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摘 要: | 目的]了解不同浓度激素下对省沽油组培芽增殖的影响。方法]研究了常用浓度区间内的6-BA以及6-BA、IAA混用对于省沽油外植体生芽率的影响。结果]当6-BA浓度为0.6mg/ml时,省沽油茎段在接种后第15d生芽率达到84%;6-BA浓度在0.4mg/ml时,接种后10d的茎尖生芽率达到56%;1mg/m]6-BA+0.20mg/mlIAA浓度茎尖生芽率达到50%,茎段生芽率达到30%。结论]省沽油组培芽增殖试验中,单独使用0.4~0.6mg/ml的6-BA可达到最好的生芽刺激效果。
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关 键 词: | 省沽油 6-BA IAA 浓度 芽增殖 生芽率 |
Effects of Different Concentration Hormones on Bud Proliferation in Tissue Culture of Staphylea bumalda |
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Institution: | TAO Xu-yi et al (Anqing Vocational and Technical College, Anqing, Anhui 246003 ) |
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Abstract: | Abstract Objective] To study effects of different concentration hormones on bud proliferation in tissue culture of Staphylea bumalda DCI Method] The effects of 6-BA in commonly used concentration range and 6-BA, IAA mixture on explant germination rate of S. bumalda werI studied. Result] When 6-BA concentration came to 0.6 mg/ml, S. bumalda stem section germination rate reach a percentage of 84 at thI 15th day; with the 6-BA at the concentration of O. 4 mg/ml, we saw a stem germination rate of 56% on the 10th day; when the IAA conce~ tration was set on 0.20 mg/ml, with a invariant percentage of 6-BA, the rata jumped up to 50%, stem section germination rate came to 30%1 Conclusion ] In the test of the bud proliferation of S. bumalda, an exclusive use of 6-BA at 0.4 - 0.6 mg/ml proved to exert the most signif"t cant stimulus effect. / |
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Keywords: | Staphylea bumalda DC 6-BA IAA Concentration Bud proliferation Budding rate |
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