榆黄蘑液体深层发酵培养基的优化研究

目的]研究榆黄蘑的深层发酵最佳培养基的制备，为榆黄磨的液体发酵培养提供参考。方法]以初选培养基为基础，通过单因素试验和正交试验，确定适合榆黄蘑菌丝体生长以及适合产生胞外多糖的优化培养基。结果]确定适合榆黄蘑菌丝体生长的优化培养基配方为：马铃薯汁20．00％、葡萄糖1．00％、蔗糖1．00％、牛肉膏0．15％、蛋白胨0．15％、NaCl0．01％、磷酸二氢钾0．15％、硫酸镁0．075％、VB0．001％。适合产生胞外多糖的优化培养基配方为：马铃薯汁20．00％、葡萄糖1．50％、蔗糖1．50％、牛肉膏0．10％、蛋白胨0．10％、NaCl0．03％、磷酸二氢钾0．15％、硫酸镁0．075％、VB,0．001％。结论]可以根据不同的目的选择不同的培养基进行榆黄蘑的深层培养。

Research on the Optimization of Submerged Fermentation Medium for Pleurotus eiorinopi E Culturing

Objective] The reference for the submerged fermentation of Pleurotus eiorinopi E was provided through the study on the preparation of the best medium for its culturing.Method] Based on the single factor experiment and orthogonal test,the optimal medium suitable for Pleurotus eiorinopi E mycelium growth and the production of exopolysaccharide was determined.Results] The optimized medium formula of Pleurotus eiorinopi E mycelium growth was as follows: the potato juice,20.00%;the glucose,1.00%;the sugar,1.0...