怀地黄基因片段及其中转座酶基因的克隆与序列分析

根据已知裂叶牵牛花PNZIP启动子碱基序列的相关信息设计引物,采用PCR方法,从怀地黄基因组中扩增出5个基因片段,回收和克隆出其中一个1 096bp的基因片段。经过NCBI对比分析,发现它是一个类似转座子的相关蛋白基因序列,含有一个完整的开放阅读框,大小为450bp,编码由150个氨基酸组成的转座酶。然后,基于该开放阅读框的碱基序列,设计另一对引物,用PCR方法,从中扩增出该完整开放阅读框的序列片段。

Cloning and Sequencing of One DNA Fragment and Its Internal Transposase Gene in Rehmannia glutinosa.f.hueichingensis (Chan et Sehih)Hsiao

A pair of primers were designed according to the base information of the promoter PNZIP in Pharbitis nil(L.) Ching using software primer 3.0.Five DNA fragments of the genomic DNA in Rehmannia glutinosa.f.hueichingensis(Chan et Sehih)Hsiao were amplified;one fragment(1 096 bp) was cloned by PCR.The result showed that it was one transposon-like protein gene sequence,including a complete ORF,450 bp in size and encoding one 150-amino acid transposase(alignment with some gene sequences in NCBI).Furthermore,the another pair of primers were designed according to the base information of the ORF and the ORF was generated completely by PCR.The results will provide significant references for cloning the functional genes from Rehmannia glutinosa.f.hueichingensis(Chan et Sehih)Hsiao and their structural and functional studies in the future.