鲫鱼卵中脂质提取方法的比较

比较了提取鲫鱼卵的脂质和磷脂的三种方法.酶水解-乙醇法提取的脂质和磷脂的颜色较浅、得率较高,提取的磷脂主要成分为磷脂酰胆碱;正己烷-乙醇-丙酮法提取总磷脂、富含固醇的中性脂和含少量固醇类的中性脂,丙酮洗涤能有效地去除磷脂中的中性脂,特别是易溶于乙醇的固醇和三萜系醇,最低限度地使用有毒有机溶剂;而Bligh-Dyer提取总脂后液液萃取的经典方法提取脂质虽也较简便,但其大量使用有毒溶剂,安全性低.此外,甲醇单溶剂洗脱,紫外检测器在线检测进行的低压柱层析,分离正己烷-乙醇分提的磷脂,制备了较高纯度的磷脂酰胆碱和磷脂酰乙醇胺.

Comparison on methods of extracting lipids from roes of Carassius auratus

The color and yield of the tipids and phospholipids were better by enzymatic hydrolysis and ethanol extraction method, with PC as the main component in the extracted phospholipids. Method of. hexane and ethanol extraction and then washing by acetone could easily and efficiently extract total phospholipids, and the neutral lipids rich in sterols and another neutral lipids with lower sterols. Acetone washing could remove the neutral lipids from the phospholipids, especially the sterol and triterpenoic alcohols which are easily dissolved in the alcohol, and only a little poisonous organic solvent was used. Though the classic method Bligh-Dyer is handy, the large amount of noxious solvent it used made it unsafe. In addition, higher purity of PC and PE are prepared from the hexane and ethanol extracted phospholipids by low pressure column chromatography with methanol as washing solvent and SPD-10A detector.