| 烟草5-磷酸脱氧木酮糖还原异构酶基因(dxr)的克隆和表达分析 |
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| 引用本文： | 朱晓宇,王景,赵二卫,姚姗姗,崔红.烟草5-磷酸脱氧木酮糖还原异构酶基因(dxr)的克隆和表达分析[J].农业生物技术学报,2011,19(2). |
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| 作者姓名： | 朱晓宇 王景 赵二卫 姚姗姗 崔红 |
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| 作者单位： | 河南农业大学烟草学院,烟草行业栽培重点实验室,郑州450002 |
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| 摘 要： | 以烟草(Nicotiana tabacum)栽培品种K326为材料,采用RT-PCR技术,克隆了萜类代谢关键酶烟草5-磷酸脱氧木酮糖还原异构酶(dxr)的cDNA片段.该基因编码区长1422 bp,编码473个氨基酸残基.利用Clustal W(1.82)和Bioedit软件,对烟草与番茄(Lycopersicon esculentum)、长春花(Catharanthus roseus)、金鱼草(A ntirrhinum majus)、薄荷(Mentha piperita)、玉米(Zea mays)、拟南芥(A rabidopsis thaliana)、念珠藻(Nostoc sp.)等物种dar基因的同源性进行分析,其氨基酸同源性分别达到93.6%、87.9%、86.3%、84.6%、84.2%、82.9%和53.5%.原核表达结果证明,该基因编码蛋白的分子量约为50 kD,与氨基酸序列估算相符合.组织表达特异性分析表明,dxr基因在烟草组织中的表达强弱为花>叶>茎>腺毛>种子>根,在花和叶片中的表达量占优势.该结果对烟草萜类代谢的分子调控和品质改良具有重要的参考价值.
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| 关 键 词： | 烟草 克隆 原核表达 组织表达 |
Cloning and Expression Analysis of l-deoxy-D-xylulose-5-phosphate Reductoisomerases Gene (dxr) in Nicotina tabacum |
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| Zhu Xiaoyu,Wang Jing,Zhao Erwei,Yao Shanshan,Cui Hong.Cloning and Expression Analysis of l-deoxy-D-xylulose-5-phosphate Reductoisomerases Gene (dxr) in Nicotina tabacum[J].Journal of Agricultural Biotechnology,2011,19(2). |
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| Authors: | Zhu Xiaoyu Wang Jing Zhao Erwei Yao Shanshan Cui Hong |
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| Institution: | Zhu Xiaoyu Wang Jing Zhao Erwei Yao Shanshan Cui Hong College of Tobacco Science,Henan Agricultural University,Key Laboratory for Cultivation of Tobacco Industry,Zhengzhou 450002,China |
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| Abstract: | Isoprenoid biosynthesis via mvalonate-independent pathway is very important to tobacco resistance and leaf quality.1-deoxy-D-Xylulose-5-phosphate Reductoisomerases(dxr) is a key enzyme in biosynthesis of isopentenyl diphosphate,which is the precusor for monoterpenoid,diterpenoid and tetratepenoid compounds.To regulate the terpenoid metabolism pathway for tobacco improvement,some important genes such as dxr should be studied firstly.In this paper,dxr gene was cloned successfully from tobacco(Nicotiana tabacu... |
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| Keywords: | dxr RT-PCR |
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