Soleris检测技术在冰淇淋中大肠菌群检测的应用研究

冰淇淋中大肠菌群的传统检测方法繁琐且费时费力，不能适应现代化大型食品企业检测工作的需求。本研究将Soleris实时光电微生物检测技术引入到大肠菌群的快速检测中，得到了冰淇淋中大肠菌群检测标准曲线LogCFU=9.5175-0.854＆#215;DT（R2=0.9717）；并用国标平板法对标准曲线进行了验证，结果表明Soleris法测得的结果与平板计数结果偏差在＆#177;0.5范围以内，具有较好的一致性，重复性验证结果RSD值在0.443%～0.501%之间。而与平板计数法相比，Soleris法具有快速的优点，可使冰淇淋产品放行时间缩短13~62h。本研究建立的大肠菌群Soleris检测法具有检测时间短、灵敏度高的优势，为冰淇淋产品的微生物检测提供了一种新的方法。

The Applications and Establishment of Soleris System in Ice Cream Coliform Bacteria Detection

The detection of coliforms acounts in ice cream by traditional GB4789.3-2010 method is not only cumbersome and time-consuming, but also unable to meet the modern needs of awful testing work in large food companies. In this study, we introduced the Soleris real-time photoelectric microbial detection technology into the rapid detection of coliforms. Based on the GB Plate method, a standard curve of Soleris method for coliforms detection was prepared and veriifed, standard curve of the coliforms detection of ice cream was：Log CFU=9.5175-0.854＆#215;DT （R2=0.9717）. All Soleris test results were in agreement with the reference plating procedures within the range of ＆#177;0.5. RSD value of repetitive veriifcation test were within the range of 0.443%, 0.501%. Compared to traditional method, the release time of ifnished products was shortened for 13 to 62 hours. In brief, with short time and high sensitivity compared to traditional method, the Soleris method greatly improves the efifciency of coliform detection and becomes a promising method for microbe detection in ice-cream products.