茶树CsGPX基因全基因组鉴定和表达分析

为明确茶树谷胱甘肽过氧化物酶(CsGPX)基因与非生物胁迫的关系,本研究利用生物信息学手段在茶树基因组中筛选得到3条CsGPX基因,对其编码蛋白理化性质、基因结构、系统进化树、顺式作用元件进行了分析。结果表明,CsGPX包含完整GPX结构域和3段保守序列,都具6个外显子。预测启动子上有参与植物激素和非生物胁迫响应的顺式作用元件。使用实时荧光定量PCR测定该基因的表达谱,发现它们在不同组织中的表达有显著差异。进一步分析表明,CsGPX被低温、ABA、盐以及干旱处理上调表达,但CsGPX2和CsGPX3的表达受低温抑制。本研究为茶树CsGPX家族基因克隆和功能验证提供基础。

Genome-wide Identification and Expression Analysis of CsGPX Gene in Tea Plant (Camellia sinensis)

To reveal the possible involvement of glutathione peroxidase genes (CsGPX) in the responses of tea plants to abiotic stresses, three CsGPX genes were identified by bioinformatics tools from the tea genome data, and the gene structures, the cis-elements on their promoters, the physiochemical characteristics of the encoded proteins and the phylogenetic trees were also studied. The results showed that all the CsGPX genes contained 6 exons and shared the gene family-specific GPX domain in addition to three other conserved domains. Cis-elements for the responses of plants to hormones and abiotic stresses were identified on the promoters. Quantitative real-time PCR analysis of the expression profiles revealed that they were significantly different in expression in different tissues. Further analysis showed that the three CsGPX genes were, in general, up-regulated by treatments of low temperature, ABA, salt and drought except that CsGPX2 and CsGPX3 were down-regulated by treatment of low-temperature. The results should have laid down a basis for further characterization of CsGPX family genes in tea plant.