基于ISSR标记的咖啡资源遗传多样性分析

采用ISSR分子标记对87份咖啡资源进行遗传多样性和亲缘关系分析。结果表明,19条ISSR引物扩增出140条带,其中多态性带107条,多态性位点为76.4%。运用UPGMA方法构建了聚类图,在遗传相似系数0.625水平下,87份资源被分为3大类。第Ⅰ类群包括了3份大粒种（Coffea liberica Bull ex Hiern）;第Ⅱ类群为中粒种咖啡（C. canephora Pierre）;第Ⅲ类群包括了全部小粒种资源（C. arabica Linne）,共83份。咖啡种质的遗传关系种间容易划分,在种的分类水平上存在遗传关系多样性,而小粒种咖啡种内遗传多样性较窄。该研究结果将为咖啡种质鉴定、分类及分子育种提供重要科学依据。表明用ISSR分子标记进行咖啡资源遗传多样性的分析是可行的。

Genetic Diversity of Coffee Germplasms by ISSR Markers

To assess the genetic diversity and the relationship of coffee germplasm accessions, 87 resources were analyzed by ISSR molecular marker technology. The results demonstrated that 140 bands were amjplified by 19 primers, among which 107 bands were polymorphic, accounting for 76.4%. Based on a clustering graph using UPGMA, 87 resources could be divided into three groups at the level of 0.625. Three accessions in C. liberica were in the first group. One accessions in C. canephora was in the second group. A total of 83 accessions in C. arabica were in the third group. There were large genetic differences among species (C. arabica, C. canephora, and C. liberica), and the small genetic differences within Coffea arabica. These results would provide an important scientific basis for pineapple germplasm identification, classification and molecular breeding of coffee. The ISSR markers was an available method to study the genetic diversity of coffee germplasm resources.