中华绒螯蟹雄性生殖系统生化组成及精子代谢

通过对中华绒螯蟹肝胰腺及生殖系统各组织生化成分和乳酸脱氢酶(LDH)活性的测定发现:总碳水化合物的含量以副性腺及输精管内容物(含精荚和精浆)较高,为33.456mg·g-1湿重和21.537mg·g-1湿重,肝胰腺、精荚及精巢的含量较低;碳水化合物中的糖原和葡萄糖含量分别以输精管内容物和肝胰腺最高,分别为0.385mg·g-1湿重和5.866mg·g-1湿重,精巢和肝胰腺中的糖原含量太低而无法检测到,精荚葡萄糖含量最低;蛋白质含量以肝胰腺最高,其余各部分含量显著低于肝胰腺;脂类含量以精巢和肝胰腺最高,其中的甘油三酯仅存于肝胰腺和精巢,且肝胰腺含量显著高于精巢。乳酸脱氢酶活性以贮精囊最高,精荚仅为中等,这可能与精荚壁的通透性有关,而精浆和肝胰腺显著低于其它组织。结果表明,中华绒螯蟹能量代谢主要物质是脂类和蛋白质;精子代谢的能量物质为碳水化合物,并由精浆提供,而其代谢的方式以厌氧代谢为主。

Biochemical composition and sperm metabolism in the reproductive system of the male, Eriocheir sinensis

Biochemical composition of male reproductive system and hepatopancreas, w ith special reference to lactate dehydrogenase( LDH) activity, was determined in the Chinese mitten-handed crab, Eriocheir sinensis . The results reveal that the glandula accessoria and luminal contents( seminal plasma along with spermatophores) are rich in carbohydrates ( 33. 456mg .. g - 1 and 21. 537mg .. g - 1 of wet t issue weight, respectively ) . The carbohydrate contents of hepatopancreas and spermatophore and testis are low . Luminal contents and hepatopancreas contain the highest level of gluco se and glycogen respectively, but spermatophore contains very low level of gluco se and in testis and hepatopancreas glycogen is too low to be detected. Protein content of hepatopancreas is higher than that of other tissues. Hepatopancreas and testis contain the higher level of lipid. Triglyceride exists in the hepatopancreas and testis only and the content of hepatopancreas is higher than that of testis. LDH activity is very high in seminal vesicle, and probably this is due to the storage of seminal substances within this region. On the contrary, spermatophores show only moderate amount and a low LDH activity detected in the seminal plasma may be due to it s leakage f rom the spermatophores. Compared with reproductive system, LDH activity of hepatopancreas is significantly low. All results show that the pattern of sperm metabolism in Eriocheir sinensis is anaerobic by utilizing carbohydrates. A high carbohydrate content of glandula accessoria suggests that spermatozoa may rely on carbohydrate reserves of seminal vesicle for its survival in the spermatheca.