| 卡瓦胡椒及胡椒的荧光AFLP分子标记研究 |
| |
| 引用本文： | 施江,辛莉,杨彦,ZHENG Xue-qin.卡瓦胡椒及胡椒的荧光AFLP分子标记研究[J].河南农业科学,2007(7):80-84. |
| |
| 作者姓名： | 施江 辛莉 杨彦 ZHENG Xue-qin |
| |
| 作者单位： | 1. 河南科技大学,农学院,河南,洛阳,471003
2. 北京鼎国生物技术有限责任公司,北京,100088 |
| |
| 基金项目： | 国家科技攻关项目
,
河南科技大学校科研和教改项目 |
| |
| 摘 要： | 对6份卡瓦胡椒材料、23份栽培胡椒和野生胡椒材料、1份不同属的草胡椒材料、1份不同科的普通烟材料,共计31份试验材料,进行荧光AFLP分子标记,研究卡瓦胡椒与胡椒及其他近缘野生种的亲缘关系。结果表明,在相似系数0.52处将31份种质划分为5个类群,第1个类群有普通烟,第2个类群有草胡椒,第3个类群是卡瓦胡椒,第4个类群有胡椒,第5个类群有山胡椒、蒌叶和蒟蒌。说明卡瓦胡椒与胡椒及其他近缘野生种的亲缘关系有一定距离,为卡瓦胡椒真伪性的分子鉴定和构建卡瓦胡椒的指纹图谱提供了基础性的工作。
|
| 关 键 词： | 卡瓦胡椒 胡椒 扩增片段长度多态性 亲缘关系 |
| 文章编号： | 1004-3268（2007）07-0080-05 |
| 修稿时间： | 2007-03-26 |
Studies on the AFLP Molecular Markers of Kava and Piper nigrum |
| |
| SHI Jiang,XIN Li,YANG Yan,ZHENG Xue-qin.Studies on the AFLP Molecular Markers of Kava and Piper nigrum[J].Journal of Henan Agricultural Sciences,2007(7):80-84. |
| |
| Authors: | SHI Jiang XIN Li YANG Yan ZHENG Xue-qin |
| |
| Institution: | 1. College of Agronomy, Henan University of Science and Technology, Luoyang 471003 ,China; 2. Beijing Dingguo Bioteehnology CO. ,LTD, Beijing 100088, China; 3. State Key Laboratory of Tropiteal Crop Bioteehnology, Haikou 571101, China |
| |
| Abstract: | The genetic relationship between Kava and Piper nigrum has been studied by the AFLP molecular markers.31 test materials were used in the paper,including 6 Kava genotypes,23 cultivated and wild peppers,1 Peperomia pellucida kunth and 1 tobacco.Based on the fluorescence-labeled AFLP,the 31 germplasms were devided into 5 different groups at 0.52 of the genetic similarity coefficient.The result showed that the genetic distance between Kava and other Piper germplasms was far,and the fluorescence-labeled AFLP was applicable to the identification and DNA fingerprint construction of Kava. |
| |
| Keywords: | Kava Piper nigrum Amplified fragment length polymorphism Genetic relationship |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
