| 超高效液相色谱法测定桔百颗粒中的橙皮苷 |
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| 引用本文： | 林仙军,周炜,陆春波,张航俊.超高效液相色谱法测定桔百颗粒中的橙皮苷[J].畜牧兽医科技信息,2014(4):27-29. |
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| 作者姓名： | 林仙军 周炜 陆春波 张航俊 |
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| 作者单位： | 浙江省兽药饲料监察所,浙江杭州310020 |
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| 摘 要： | 本试验建立了超高效液相色谱法测定桔百颗粒中橙皮苷含量的方法.样品经甲醇提取,定容,采用超高效液相色谱分离,以保留时间定性,以峰面积定量.橙皮苷在浓度2 ～ 100μg/mL范围内线性良好,相关系数r为1.0000.平均加样回收率为98.2％,RSD为1.4％.该方法准确、可靠,为桔百颗粒的质量控制和评价提供了依据.
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| 关 键 词： | 桔百颗粒 橙皮苷 超高效液相色谱 测定 |
Determination of Hesperidin in the Jiebai Keli by UPLC |
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| Lin Xianjun,Zhou Wei,Lu Chunbo,Zhang Hangjun.Determination of Hesperidin in the Jiebai Keli by UPLC[J].Chinese Journal of Animal Husbandry and Veterinary Medicine,2014(4):27-29. |
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| Authors: | Lin Xianjun Zhou Wei Lu Chunbo Zhang Hangjun |
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| Institution: | ( Zhejiang Province Institute of Veterinary Drug ＆ Feedstuff Control, Hangzhou 310020,China ). |
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| Abstract: | A method was established for determining ofhesperidin in Jiebai Keli Chinese herbal medicine by ultra performance liquid chromatography （UPLC）. The hesperidin was extracted fi＇om samples by methanol, separated by UPLC. it＇s characteristics were idenfied according to the retaining time and it was quantified according the area of its peak. The results showed that the calibration curve was linear （r=1.0000） over the range of 2.0-100.0 μg/mL. The average recovery rate was 98.2% and the intraassay CV was 1.4%. This method was repeatable and veracious, and it was well suitable for the product quality inspection and control for Jiebai Keli. |
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| Keywords: | hesperidin Jiebai Keli UPLC |
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