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草铵膦对大豆胚尖不定芽形成的影响
引用本文:王萍,管娟娟,冯远航,王罡,季静. 草铵膦对大豆胚尖不定芽形成的影响[J]. 作物杂志, 2011, 27(6): 60-63
作者姓名:王萍  管娟娟  冯远航  王罡  季静
作者单位:1. 淮海工学院海洋学院,222005,江苏连云港
2. 淮海工学院海洋学院,222005,江苏连云港;天津大学农业与生物工程学院,300072,天津
3. 天津大学农业与生物工程学院,300072,天津
基金项目:国家转基因生物新品种培育重大专项
摘    要:
本文以大豆黑农37、吉育91和淮豆4号3种基因型的胚尖为外植体,研究草铵膦7种浓度对大豆不同基因型胚尖不定芽形成的影响。结果表明,大豆3种基因型表现出对草铵膦的耐性不同。吉育91耐性较强,淮豆4号耐性较差。依大豆基因型的不同,草铵膦浓度在0.6mg/L或1.2mg/L时抑制大豆胚尖不定芽形成。本研究为以Bar基因作为选择标记的大豆胚尖遗传转化奠定了基础。

关 键 词:大豆  胚尖  不定芽  草铵膦  

Effects of Glufosinate on Formation of Adventitious Buds from Embryonic Tip of Soybean
Wang Ping,Guan Juanjuan,Feng Yuanhang,Wang Gang,Ji Jing. Effects of Glufosinate on Formation of Adventitious Buds from Embryonic Tip of Soybean[J]. Crops, 2011, 27(6): 60-63
Authors:Wang Ping  Guan Juanjuan  Feng Yuanhang  Wang Gang  Ji Jing
Affiliation:Wang Ping1,Guan Juanjuan1,Feng Yuanhang1,2,Wang Gang2,Ji Jing2(1School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang 222005,Jiangsu,2School of Agriculture and Biological Engineering,Tianjin University,Tianjin 300072,China)
Abstract:
Embryonic tips of soybean genotypes including Heinong 37,Jiyu 91 and Huaidou 4 were used as the explants to study effects of glufosinate on formation of adventitious buds from the different genotypes of soybean's embryonic tips.The results showed that different tolerance to glufosinate existed among the three genotypes involved,in which the highest tolerance was observed in Jiyu 91.The formation of adventitious buds from the soybean embryonic tips was inhibited by glufosinate at the concentration of 0.6mg/L...
Keywords:Soybean  Embryonic tips  Adventitious bud  Glufosinate  
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