| 番茄双抗TMV和CMV基因工程的研究 |
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| 引用本文： | 毕玉平,廖俊杰.番茄双抗TMV和CMV基因工程的研究[J].山东农业科学,1997(6):4-5. |
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| 作者姓名： | 毕玉平 廖俊杰 |
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| 摘 要： | 在ＴＭＶ外壳蛋白（ＣＰ）基因的５′和３′端分别合成寡聚核苷酸引物Ｐ１和Ｐ２，并分别引入ＣｌａＩ和ＢａｍＨＩ位点，采用ＰＣＲ技术扩增ＴＭＶＣＰ基因，用ＣｌａＩＢａｍＨＩ消化后重组到ｐＢｌｕｅ ｓｃｒｉｐｔＫＳ＋中，并将该基因与ＣＭＶＣＰ基因重组在同一个表达载体ｐＥ３上获得双价ＣＰ基因表达载体ｐＥＴＣ２，转化番茄，获得再生植株。通过点杂交、ＰＣＲ检测和Ｓｏｕｔｈｅｒｎ杂交，证实２、１２和１３号为转ＴＭＶ和ＣＭＶＣＰ基因的工程植株。工程植株在温室中表现正常，比对照植株表现出明显的抗性。
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| 关 键 词： | 番茄，烟草花叶病毒，黄瓜花叶病毒，外壳蛋白基因，抗病毒基因工程 |
Study on Transgenic Tomato Plants Expressing Resistance to Cucumber Mosaic Virus and Tobacco Mosaic Virus |
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| Abstract: | おMV coat protein (CP) gene and TMV CP gene cloned were recommbinated into the expressing vector pE3,then transfered into tomato plants.Three transgenic tomato plants(No.2,12,13)were selected by the means of dot blotting,PCR and Southern blotting.The transgenic tomato plants grow strongly comparing with control plants in the green house,and show resistance to CMV and TMV obviously. |
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| Keywords: | Tomato CMV TMV CP gene gene engineering resistance to virus |
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