与黄瓜抗炭疽病相关基因连锁的AFLP标记的筛选

以黄瓜抗炭疽病母本66和感炭疽病父本A18及其F₂代分离群体为试材, 采用BSA法和AFLP技术建立了对炭疽病的抗病组和感病组, AFLP引物组合E24M48在抗感组间表现多态性, 且呈共显性。经220个F₂单株分析, 在高抗单株和高感单株中分别仅扩增出251 bp和245 bp的特异片段, 而在中间类型个体中同时扩增出了该两个特异片段。连锁值测定结果表明, 该特异带与黄瓜炭疽病抗病相关基因紧密连锁,距离为21727 cM。测序结果显示, 该两个片段的大小分别为251 bp和245 bp, 并且两个片段的差异在于两个“TCT”重复序列(6个碱基) 的插入或缺失。

AFLP Markers of Cucumber Anthracnose Resistance-related Gene

With a F₂ population derived from 66 ×A18 asmaterials, polymorphism between resistant andsuscep tible bulk of cucumber anthracnose were studied using BSA method and AFLP technology. A codominantAFLP marker, E24M48=251 bp /245 bp, was screened. The resistant and suscep tible F₂ plants possessedthe 251 bp and the 245 bp fragment, respectively, and the mid-plants owned the two fragments. The AFLPmarkerwas closely linked with the cucumber anthracnose resistance related gene, and the genetic distance between the marker and gene was 21727 cM. Fragements sequencing of the codominantAFLP marker indicatedthat their length were 251 bp and 245 bp, respectively. The difference of the two fragements lied in the insertdeletion of two “TCT” repeat sequences.