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| 响应面法优化灰树花液体菌种配方研究 | |
| 引用本文： | 潘辉,郭倩,刘朝贵,李正鹏,李玉,周峰,于海龙,王瑞娟. 响应面法优化灰树花液体菌种配方研究[J]. 上海农业学报, 2011, 27(2) |
| 作者姓名： | 潘辉 郭倩 刘朝贵 李正鹏 李玉 周峰 于海龙 王瑞娟 |
| 作者单位： | 1. 西南大学园艺园林学院,重庆市蔬菜学重点实验室,重庆400715 2. 国家食用菌工程技术研究中心,农业部应用真菌资源与利用重点开放实验室,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201106 3. 上海海洋大学食品学院,上海,200090 |
| 基金项目： | 上海市农委重点攻关项目[沪农科攻字(2009)第2-2号]; 上海市农业科学院开发基金[农科发2008(03)]资助 |
| 摘 要： | 以灰树花(Grifola frondosa)菌丝生物量为指标,采用响应面法优化工厂化栽培灰树花液体菌种发酵培养基配方。通过Plackett-Burman设计得出麸皮和玉米粉是影响灰树花菌丝生物量的主效应因素,用最陡爬坡试验逼近最大响应区域,再进行中心组合设计试验及响应面法分析,得到最佳配方为麸皮38.48g/L、玉米粉48.92g/L,对应灰树花液体菌种最大生物量为49.25g/L。 |
| 关 键 词： | 灰树花 响应面法 优化配方 生物量 |
Optimizing the medium for liquid inoculation of Grifola frondosa by response surface analysis |
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| PAN Hui,GUO Qian,LIU Chao-gui,LI Zheng-peng,LI Yu,ZHOU Feng,YU Hai-long,WANG Rui-juan. Optimizing the medium for liquid inoculation of Grifola frondosa by response surface analysis[J]. Acta Agriculturae Shanghai, 2011, 27(2) | |
| Authors: | PAN Hui GUO Qian LIU Chao-gui LI Zheng-peng LI Yu ZHOU Feng YU Hai-long WANG Rui-juan |
| Affiliation: | PAN Hui~1,GUO Qian~2,LIU Chao-gui~1,LI Zheng-peng~3,LI Yu~2,ZHOU Feng~2,YU Hai-long~2,WANG Rui-juan2 (1 Chongqing Key Laboratory of Vegetables,College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China,2 National Engineering Research Center of Edible Fungi,Key Laboratory of Applied Mycological Resources and Utilization,Ministry of Agriculture,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Institute of Edible Fungi,SAAS,Shanghai 201106,3 College of F... |
| Abstract: | Based on the mycelium biomass index the composition optimization of main medium for the liquid inoculation of factory-cultured Grifola frondosa was conducted by response surface analysis. It was found from the Plackett-Burman design that wheat bran and corn meal were two major factors influencing the biomass,then the maximum response range was approached by using the steepest ascent experiment,and lastly it was concluded by the central composite design and the response surface analysis that the optimal medi... |
| Keywords: | Grifola frondosa Response surface analysis Optimized medium Biomass |
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