Pb2+胁迫下Ca2+对板蓝根幼苗生理特性的影响

以板蓝根为材料,研究了Pb2+胁迫下Ca2+对板蓝根幼苗POD、SOD活性、根系活力及蛋白质含量的影响。结果表明:在Pb2+处理下,板蓝根幼苗POD、SOD活性及蛋白质含量随Pb2+浓度升高呈先上升后下降的趋势,根系活力显著下降;在Ca2+处理下,浓度为80、160mg/L时,板蓝根幼苗POD、SOD活性、根系活力及蛋白质含量显著升高,浓度为320mg/L时,则显著低于对照,有明显抑制作用;Ca2+、Pb2+混合处理,80、160mg/LCa2+显著提高了Pb2+胁迫下POD、SOD活性、根系活力及蛋白质含量,具有缓解Pb2+毒害的作用,Ca2+为320mg/L时,与Pb2+起协同胁迫作用,增强对板蓝根幼苗的毒害效应。

The Effect of Ca2+ on Radix Isatidis Seedling Physiological Characteristics under Pb2+ Stress

With Radix Isatidis as tested materials,the activities of SOD and POD,the root activities and protein content were studied by treating the radix isatidis seedlings with different concentration of Ca2+ and Pb2+.The results showed that by treating the seedlings only with Pb2+,the activity of SOD and POD and the protein content increased following with the increasing of Pb2+ concentration at first,then decreased with the root activities decreased significantly.Treating the seedlings with Ca2+ at the concentration of 80 and 160 mg/L,the activities of SOD,POD and the protein content significantly increased,for Ca2+ treatment at concentration of 320mg/L,every physiological index was significantly lower than the contrast,meaning that this concentration had significant inhibitory effect;Treating the seedlings both with Ca2+ and Pb2+,the Pb2+ induced injuries could be ameliorated by Ca2+,decreased the inhibitive effect of Pb2+ on Radix Isatidis seedlings,and increased the content of protein、the root activities and the activity of SOD and POD.However,when the Ca2+ was at concentration of 320mg/L,enhanced stress effect of Pb2+ and mischief effect on root growth were observed.