水分胁迫下甜菜幼苗过氧化物酶cprx1基因的半定量表达模式分析

为明确甜菜过氧化物酶cprx1基因在抗旱节水中的功能,利用甜菜品种HI0466(抗旱性较强)、农大甜研4号(抗旱性较弱)为材料,通过已克隆的甜菜cprx1基因序列设计引物,利用半定量RT-PCR方法,对甜菜幼苗根系、叶片中cprx1基因在正常供水及PEG6000模拟水分胁迫1d、3d、5d及复水1d、2d时的表达模式进行分析。结果显示,2个甜菜品种幼苗根、叶中cprx1基因在正常供水情况下均有一定量的表达;在水分胁迫1d均诱导上调表达;水分胁迫至第3天HI0466根、叶中表达量显著增强,而农大甜研4号根、叶中该基因表达受到抑制;胁迫至第5天HI0466根、叶中该基因仍有微量表达,而农大甜研4号根、叶内该基因表达接近停止;复水2d后表达量均恢复至胁迫前水平。

Expression Analysis of Sugar Beet Seedlings Peroxidase cprx1 Gene during Water Stress and Re-watering by Semi-quantitative RT-PCR

In order to study the functions of Sugar beet seedlings peroxidase cprx1 gene in drought tolerance and water use efficiency,the semi-quantitative RT-PCR was used to investigate the expression patterns of cprx1 gene in the root and leaves of sugar beet seedling of HI0466(more drought tolerance)and Nongdatianyan4(less drought tolerance)during PEG 6000 simulated water stress and re-watering after serious water stress.The results showed that the cprx1 gene has already expressed in the root and leaves of sugar beet seedling of HI0466 and Nongdatianyan4 during normal water condition.The expressions in the root and leaves of sugar beet seedling of cprx1 gene were up-regulated at 1d of 20% PEG 6000 simulated water stress,then were up-regulated constantly in HI0466 and were down-regulated in Nongdatianyan4 at 3d of 20% PEG 6000 simulated water stress.After 5d of 20% PEG 6000 simulated water stress,A few expressions appeared in HI0466,However,disappeared in Nongdatianyan4,increased again after 2d of re-watering to the normal level.