| 地衣芽孢杆菌16S-ITS标记及其特异性分析 |
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| 引用本文： | 陈冲,佟建明,张潞生.地衣芽孢杆菌16S-ITS标记及其特异性分析[J].安徽农业科学,2011(14):8206-8207. |
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| 作者姓名： | 陈冲 佟建明 张潞生 |
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| 作者单位： | 山西农业大学信息学院;中国农业科学院北京畜牧兽医研究所;中国农业大学农学与生物技术学院; |
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| 摘 要： | 目的]建立地衣芽孢杆菌的分子鉴定方法。方法]通过对地衣芽孢杆菌TS-01的16S和ITS序列进行克隆测序和差异性分析,以该区序列为靶序列设计地衣芽孢杆菌TS-01的特异性引物,并用该引物扩增全部受试菌种。结果]从ITS和16S rDNA区间设计了地衣芽孢杆菌种特异性引物,特异性引物PCR的最佳退火温度为67.2℃,循环数为24个;地衣芽孢杆菌TS-01可扩增出1条905 bp的标记片段,其他受试菌株均为阴性,从而证明试验得到了在种水平上对该菌种进行准确鉴定的特异16S-ITS标记。结论]地衣芽孢杆菌TS-01分子鉴定方法的建立,为地衣芽孢杆菌的分子诊断奠定了基础。
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| 关 键 词： | 地衣芽孢杆茵 特异引物 16S ITS |
Analysis on 16S-ITS Marker from Bacillus licheniformis and Its Specificity |
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| CHEN Chong et al.Analysis on 16S-ITS Marker from Bacillus licheniformis and Its Specificity[J].Journal of Anhui Agricultural Sciences,2011(14):8206-8207. |
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| Authors: | CHEN Chong |
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| Institution: | CHEN Chong et al(Information College of Shanxi Agricultural University,Taigu,Shanxi 030801) |
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| Abstract: | Objective]The study aimed to establish the molecular identification methods of Bacillus licheniformis.Method]Through the cloning sequencing test and the difference analysis on 16S and ITS sequences of B.licheniformis TS-01,the specific primer of B.licheniformis TS-01 was designed with the sequence of the above regions as the target sequence and the whole tested strains were amplified by using this specific primer.Result]The specific primers of B.licheniformis were designed resp.from the DNA section of 16... |
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| Keywords: | Bacillus licheniformis Specific primer 16S ITS |
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