|
|||||
|
|
| ISSR-PCR分子标记法鉴别宁杞1号与雄性不育枸杞 | |
| 引用本文： | 思彬彬,王镇. ISSR-PCR分子标记法鉴别宁杞1号与雄性不育枸杞[J]. 安徽农业科学, 2011, 39(14): 8309-8310 |
| 作者姓名： | 思彬彬 王镇 |
| 作者单位： | 北方民族大学生物科学与工程学院,宁夏银川,750021;北方民族大学生物科学与工程学院,宁夏银川,750021 |
| 基金项目： | 北方民族大学资助项目(2007Y049) |
| 摘 要： | [目的]探索在核酸分子水平上鉴别道地药材宁夏枸杞中不同的品种。[方法]从50条ISSR引物中筛选合适的引物对宁杞1号与雄性不育2个品种进行PCR扩增及电泳分析,寻找特征位点。[结果]1条ISSR引物扩增出较为明显的多态性特征条带,可用于宁杞1号与雄性不育的鉴别。[结论]ISSR-PCR作为一种简便、可靠的分子标记方法,可用于枸杞的鉴别。 |
| 关 键 词： | 枸杞(Lycium barbarum L.) ISSR 鉴别 |
Identification of No.1 and Male Sterility of Lycium barbarum L.by ISSR-PCR Molecular Marker Technique |
|
| SI Bin-bin et al. Identification of No.1 and Male Sterility of Lycium barbarum L.by ISSR-PCR Molecular Marker Technique[J]. Journal of Anhui Agricultural Sciences, 2011, 39(14): 8309-8310 | |
| Authors: | SI Bin-bin et al |
| Affiliation: | SI Bin-bin et al(College of Bioscience and Engineering,Northern University for Nationalities,Yinchuan,Ningxia 750021) |
| Abstract: | [Objective]To identify genuine medicinal materials of Lycium barbarum L.by ISSR-PCR molecular marker technique on the level of DNA molecular.[Method] Suitable primers were screened out from 50 ISSR primers(Inter-simple sequence repeat),and then used in PCR amplification and electrophoretic analysis for distinguishing Lycium barbarum L.from No.1 and male sterility by finding specific sites.[Result]One ISSR primer could amplify polymorphic bands that were suitable for distinguishing Lycium barbarum L.from No.... |
| Keywords: | Lycium barbarum L. ISSR Identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |