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| 甜瓜属REMAP分子标记体系的建立及应用 | |
| 引用本文： | 王东,江彪,娄群峰,陈劲枫. 甜瓜属REMAP分子标记体系的建立及应用[J]. 中国瓜菜, 2011, 24(3): 6-9 |
| 作者姓名： | 王东 江彪 娄群峰 陈劲枫 |
| 作者单位： | 作物遗传与种质创新国家重点实验室·农业部南方蔬菜遗传改良重点开放实验室·南京农业大学,南京,210095 |
| 基金项目： | 国家自然科学基金重点项目，教育部高等学校博士学科点专项科研基金项目，江苏省科技支撑计划 |
| 摘 要： | 基于甜瓜属Ty1-copia类逆转座子的长末端重复序列信息,结合ISSR引物序列,在体系优化的基础上建立了适用于甜瓜属物种的REMAP(retrotransposon-microsatellite amplified polymorphism)标记体系。对46份甜瓜属不同类型材料进行聚类分析,证明该标记体系能有效检测甜瓜属不同类型材料间的多态性,可以用于甜瓜属作物品种鉴定及指纹图谱构建。 |
| 关 键 词： | 甜瓜属 分子标记 REMAP 多态性 |
Development and Application of REMAP Molecular Marker System in Cucumis |
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| WANG Dong,JIANG Biao,LOU Qun-feng,CHEN Jin-feng. Development and Application of REMAP Molecular Marker System in Cucumis[J]. China Cucurbits And Vegetables, 2011, 24(3): 6-9 | |
| Authors: | WANG Dong JIANG Biao LOU Qun-feng CHEN Jin-feng |
| Affiliation: | WANG Dong,JIANG Biao,LOU Qun-feng,CHEN Jin-feng(State Key Laboratory of Crop Genetics and Germplasm Enhancement · Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture·Department of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu,210095,China) |
| Abstract: | REMAP(retrotransposon-microsatellite amplified polymorphism) marker system was developed based on the long terminal repeat(LTR) sequence of Ty1-copia retrotransposon in Cucumis and combination of the ISSR primer sequence. Forty-six cultigens in Cucumis were genotyped using REMAP markers and the results indicated REMAP can efficiently detect polymorphism among different melon material. Thus it can be used for cultivar identification and fingerprinting in Cucumis. |
| Keywords: | Cucumis Molecular marker REMAP Polymorphism |
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