GnRH脉冲对体外原代培养大鼠GTH细胞中 LHβ mRNA表达水平的影响

研究不同脉冲频率GnRH对GTH细胞分泌LH的影响。利用细胞体外培养技术结合荧光定量PCR技术,对体外培养的大鼠原代垂体细胞在不同脉冲频率GnRH刺激下,细胞LHβ mRNA的变化进行了研究。在相同振幅条件下,高频刺激(30 min间隔)时,LHβ mRNA表达水平达到高峰。结果表明,高频脉冲主要引起LH的表达,支持了GnRH脉冲频率本身就是一个调控信号的假说。

The Influence of GnRH Impulse on the Expression of LHβ mRNA in Rats' GTH Cells Primarily Cultured in vitro

To explain at molecular level the influence of GnRH at different impulse frequencies on the secretion of LH by GTH.Research the change of LHβ mRNA in cells when the pituicytes of original generation of rats cultured in vitro were stimulated by GnRH at different impulse frequencies, by applying the technique of culture in vitro of cells in combination with the fluorescent quantitation of PCR.The result showed that in the condition of the same vibration amplitude, when the cells were stimulated by impulse at high frequency (at the interval of 30 min), the level of LHβ mRNA reached the top.This supports the hypothesis that GnRH impulse frequency itself is a regulation signal,and GnRH impulse at high frequency brings about mainly the response of LHβ.