基于SSR标记的山西省辽东栎自然居群遗传多样性分析

利用SSR分子标记对山西省8个辽东栎天然群体进行遗传多样性分析，并与北京东灵山小龙门林场群体进行地理距离和遗传距离相关关系的对比分析，旨在为辽东栎遗传多样性保护提供一定的依据。研究使用11对SSR引物进行遗传多样性分析，得到平均等位基因数为10.272 7，平均有效等位基因数为5.185 9；得出山西省辽东栎群体的平均期望杂合度为0.753 8，Nei多样性指数为0.752 1，Shannon多样性指数为1.754 3。这一结果表明辽东栎的遗传多样性水平是相对较高的。研究还表明，辽东栎群体的绝大部分遗传变异是发生在群体内的（95.99%），仅有4.01%的遗传变异发生在群体间，并且遗传距离和地理距离之间具有显著的正相关关系（r=0.752 2，P0.05）。 

Genetic diversity in natural populations of Quercus liaotungensis in Shanxi Province based on nuclear SSR markers

The research used SSR markers to analyze the genetic diversity of eight natural Quercus liaotungensis populations in Shanxi Province,and the group of Dongling Mountain in Beijing was selected to compare the relationship of geographic distance and genetic distance.The aim is to present a basis for protecting the genetic diversity of Q.liaotungensis.Eleven SSR markers were used to analyze the genetic diversity,and the results were:average number of alleles was 10.272 7,average number of effective number of alleles was 5.185 9,mean expected heterozygosity was 0.753 8,Nei diversity index was 0.752 1,and the Shannon diversity index was 1.754 3.The results proved that the genetic diversity of natural Q.liaotungensis populations was at a higher level.Moreover,the research showed that the majority of genetic variation occurred within populations(95.99%),and only 4.01% occurred among the populations.There was a significant correlation between genetic distance and geographical distance(r=0.752 2,P<0.05).