Fibronectin supports endotoxemia mice survival |
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Authors: | WU Yong CHEN Yuan-zhong Lü Lian-huang |
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Institution: | Fujian Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, China |
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Abstract: | AIM:To study the preventive effect of fibronectin on hepatic failure induced by endotoxin in mice.METHODS:The survival rate was observed in endotoxemia mice injected with fibronectin from human plasma. The tissue damage and expression of TNFα, IL-1β, IL-6 mRNA in hepatocyte were detected by the methods of histology, ultrastructure, DNA fragementation and RT-PCR.RESULTS:①Fibronectin obviously reduced the mortality of endotoxemia mice sensitized by D-galactosamine(GalN). ②Histopathology showed that less necrosis occurred on the hepatocyte of endotoxemia mice injected with fibronectin, compared with saline control. ③Ultrastructure and DNA fragmentation showed fibronectin suppressed hepatocyte apoptosis induced by LPS. ④Fibronectin down-regulated the overexpression of TNFα, IL-1β, IL-6 mRNA on hepatocyte induced by LPS.CONCLUSION:Fibronectin supports endotoxemia mice surrival by down-regulating the expression of TNFα, IL-β, IL-6, it may be a potent therapy for endotoxemia. |
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Keywords: | Fibronectins Endotoxemia Cytokines Apoptosis |
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