Effect of L-arginine on function and structure of mitochondria in ischemia-reperfusion myocardial cells in rabbits |
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Authors: | WANG Wan-tie XU Tao XU Zheng-jie JIN Ke-ke PAN Xue-rong LI Dong FANG Zhou-xi |
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Institution: | 1. Department of Pathophysiology, Wenzhou Medical College, Wenzhou 325027, Zhejiang, China;
2. Department of Pathology Anhui Medical College of Chinese Medicine, Hefei 230038, China;
3. Centre of Analysis, Clinical Laboratory Medicine, Wenzhou Medical College, Wenzhou 325027, Zhejiang, China;
4. Department of Electron Microscope, Wenzhou Medical College, Wenzhou 325027, Zhejiang, China |
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Abstract: | AIM: To study the effect of L-arginine (L-Arg) on function and structure of mitochondria in ischemia-reperfusion (MRI) myocardial cells. METHODS: Thirty rabbits were randomly divided into three groups (n=10 in each), control group, MIR group and MIR+L-Arg group. The mitochondrial respiratory function, Ca2+ concentration (Ca2+]m), malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were determined. Meanwhile, the contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), total adenylic acid number (TAN) and energy charge (EC) in the myocardial tissue were respectively measured. Moreover, the ultrastructure changes in myocardial mitochondria were observed during MIR. RESULTS: The mitochondrial respiratory control rate (RCR), velocity 3 (V3), SOD, surface density (Sv) and specific surface (δ) in MIR+L-Arg group were higher than those in MIR group, velocity 4 (V4), Ca2+]m, MDA, volume density (Vv), horizental diameter (Hd) were lower than those in MIR group. ATP, ADP, TAN and EC levels of myocardial tissue were higher than those in MIR group. There was no significant difference between MIR+L-Arg and control group in V3, V4, SOD, MDA, Vv, Sv, δ, Nv, Vd, AMP and TNA. CONCLUSION: It is suggested that L-Arg improves the function and structure of mitochondria in myocardial cells in the reperfusion injury after myocardial ischemia by decreasing oxygen free radical level and Ca2+ overload in the mitochondria. |
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Keywords: | Myocardial ischemia Mitochondria heart Arginine |
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