Human interleukin-1β induces A375-S2 human melanoma apoptosis through caspase pathway |
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Authors: | WANG Che WANG Min-wei TASHIRO Shin-ichi ONODERA Satoshi IKEJIMA Takashi |
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Institution: | 1. China-Japan Research Institute of Medical and Pharmaceutical Sciences,Shenyang Pharmaceutical University,Shenyang 110016,China;
2. Department of Clinical and Biomedical Sciences,Showa Pharmaceutical University,Tokyo 194-8543,Japan |
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Abstract: | AIM: To study the molecular biological mechanism and signal transduction pathway of interleukin-1β (IL-1β)-induced apoptosis in A375-S2 melanoma cells. METHODS: Photomicrocropy showed typical apoptotic changes. The cytotoxic effect of IL-1β in vitro and influences of caspases in this effect were measured by MTT assay. The cytotoxicity of cells was assessed by LDH-based assay. Degradation of DNA was detected by agarose gel electrophoresis. RESULTS: The inhibitory effect of IL-1β on A375-S2 cell growth was in a dose and time-dependent manner, and cell death rate reached more than 90% at 72 h after treatment with 10-9mol/L IL-1β. The inhibitors of caspase-family, -1, -3, -8, -9, and -10, partially blocked cell death at early stage. LDH assay showed that major IL-1β-induced cell death was apoptosis, and in a dose and time-dependent manner. Typical apoptotic DNA ladder was observed in agarose gel electrophoresis. CONCLUSION: IL-1β induced apoptosis in melanoma A375-S2 cells by activating caspase pathway. |
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Keywords: | Interleukin-1 Melanoma A375-S2 cells Apoptosis Caspases |
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