Protective effect of HSP70 on injuried myocardial cells induced by H2O2 |
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| Authors: | ZHANG Wei-hua WANG Li-na LI Quan-feng XU Chang-qing |
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| Institution: | Department of Pathophysiology, Harbin Medical University, Harbin 150086, China |
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| Abstract: | AIM: To investigate the role of heat-shock protein 70(HSP70) in the protection of myocardial cells against ischemic injury.METHODS: Myocardial cells were cultured in vitro. HSP70 was induced by hyperthermia. H2O2-injured myocardial cells were divided into different groups. Flow cytometry, DNA Ladder and biochemistry methods were employed to observe the myocardial cells of different groups.RESULTS: Immunohistochemistry showed hyperthermia induced the up-regulation of HSP70 in myocardial cells. Apoptotic rate, activity analysis of cytochrome C and succinic dehydrogenase in H2O2-injuried and HSP70-protected groups were obviously different. Electron micrograph shomed hyperthermia alliviated myocardial cell injury induced by H2O2. CONCLUSION: HSP70 delays apoptosis and protects against H2O2-induced myocardial cell injury. |
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| Keywords: | Heat-shock proteins 70 Hydrogen peroxide Apoptosis Myocardium Rats |
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