Inhibition of cell proliferation and arrest of cell cycle progression by blocking Cl- channels in nasopharyngeal carcinoma cells |
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Authors: | WANG Li- wei CHEN Li-xin ZHU Lin-yan MAO Jian-wen NIE Si-huai ZHANG Jin ZHONG Ping CAI Bo LI Pan |
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Institution: | 1. Department of Physiology, Guangdong Medical College, Zhanjiang 524023, China;
2. Laboratory of Cell Biology, Guangdong Medical College, Zhanjiang 524023, China |
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Abstract: | AIM: The roles of Cl-channels in regulatory volume decrease (RVD), cell proliferation and cell cycle progression in nasopharyngeal carcinoma cells (CNE-2Z) were investigated. METHODS: Image analysis of living cells was used to detect the volume changes following exposure to hypotonic solutions. Cell viability was determined by the trypan blue assay. MTT method was applied to detected cell proliferation. The effect of the blocker on the cell cycle distribution was monitored by the flow cytometry. RESULTS: 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) inhibited RVD and cell proliferation in a dose-dependent manner. NPPB at the concentration of 100 μmol/L arrested cells in G1 phase (G1 population increased from 54% to 71% at 48 h after treatments), but did not significantly alter cell viability. CONCLUSION: Block of chloride channels suppressed cell proliferation by arresting cells in G1 phase. The results suggest that activation of Cl-channels and RVD is necessary for facilitating cells to proceed to the S phase from G1 phase and maintaining cell proliferation. |
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Keywords: | Nasopharyngeal neoplasms Tumor cells Chloride channels Cell cycle |
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