Expression of CD40-CD40L during rat myocardial ischemia-reperfusion injury

AIM:To investigate the expression of CD40 and CD40 ligand(CD40L) during myocardial ischemia/reperfusion injury in rats.METHODS:In the rat modal with myocardial ischemia/reperfusion(MI/R),the changes of CD40 and CD40L expression in blood and myocardial tissues were detected by flow cytometry and immunohistochemistry, respectively. There were seven animal groups in the study, including the normal group (n=3), the ischemia 30 min group(I_{30 min},n=6),the ischemia/reperfusion 1 min,5 min,10 min,20 min and 30 min groups(each group, n=6).RESULTS:The indexes of the expression of CD40 and CD40L in I_{30 min} group were higher than those in control group,(P＜0.05); the indexes of R_{5 min},R_{10 min} groups were higher than those in the I_{30 min} group (P＜0.05);the indexes were the highest in R_{5 min} and then decreased in R_{10 min},R_{20 min},R_{30 min} group. The immunohistochemical results revealed that. CD40-CD40L expression increased on the cardiocyte membraneduring the ischemia/reperfusion injury.CONCLUSION:CD40 and CD40L may participate in the development of myocardial ischemia/reperfusion injury.