| 杂交鲶精子入卵扫描电镜观察 |
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| 引用本文： | 陈军,刘伟,赵春刚,王丙乾,潘伟志. 杂交鲶精子入卵扫描电镜观察[J]. 吉林农业大学学报, 2004, 26(3): 343-346 |
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| 作者姓名： | 陈军 刘伟 赵春刚 王丙乾 潘伟志 |
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| 作者单位： | 中国水产科学研究院黑龙江水产研究所,哈尔滨,150070;中国水产科学研究院黑龙江水产研究所,哈尔滨,150070;中国水产科学研究院黑龙江水产研究所,哈尔滨,150070;中国水产科学研究院黑龙江水产研究所,哈尔滨,150070;中国水产科学研究院黑龙江水产研究所,哈尔滨,150070 |
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| 基金项目： | 科技部农业科技成果转化基金资助项目(02EFN216900727) |
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| 摘 要： | 采用扫描电镜观察杂交鲶(索氏六须鲶♀鲶♂)精、卵形态及精子入卵过程。结果证实：异源的鲶鱼精子能够按照正常的受精细胞学程序进入到索氏六须鲶的卵内并与之受精。精卵相遇3s时，精子就能迅速到达精孔区，15S时受精孔打开，1min内完成精子入卵，15min受精孔封闭，20min精子全部解体。
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| 关 键 词： | 索氏六须鲶 鲶 精子 卵 扫描电镜 |
| 文章编号： | 1000-5684(2004)03-0343-04 |
Observation of the Entrance Process of Hybrid Silurus asotus Sperm into Eggs by Scanning Electron microscope |
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| CHEN Jun,LIU Wei,ZHAO Chun-gang,WANG Bing-qian,PAN Wei-zhi. Observation of the Entrance Process of Hybrid Silurus asotus Sperm into Eggs by Scanning Electron microscope[J]. Journal of Jilin Agricultural University, 2004, 26(3): 343-346 |
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| Authors: | CHEN Jun LIU Wei ZHAO Chun-gang WANG Bing-qian PAN Wei-zhi |
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| Abstract: | The scanning electron microscope was applied to observe the morphology of sperms and eggs of Hybrid Silurus asotus and Silurus soldatov as well as the process in which its sperms penetrated eggs. The results of the hybrid between Silurus asotus and Silurus soldatov showed that sperms of Silurus asotus could get into eggs of Silurus soldatov and made it fertilized. It had the similar process of the fertilization with the species of Silurus asotus. At 3 s after fertilization, the sperms entered the micropyle, at 15 s after fertilization, the micropylar canal opened; the fertilization was finished in 1 min; the micropylar canal closed in 15 min after fertilization; all of the sperms outside the micropyle disorganized in 20 min. |
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| Keywords: | Silurus soldatov Silurus asotus sperm egg scanning electron microscope |
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