| 球孢白僵菌分离培养基的筛选及应用 |
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| 引用本文： | 王韬远,张正坤,徐文静,王义生,赵宇,王金刚,李启云.球孢白僵菌分离培养基的筛选及应用[J].中国农学通报,2013,29(9):134-138. |
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| 作者姓名： | 王韬远 张正坤 徐文静 王义生 赵宇 王金刚 李启云 |
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| 作者单位： | 1. 东北农业大学园艺学院,哈尔滨,150030
2. 吉林省农业科学院植物保护研究所/农业部东北农作物有害生物综合治理重点试验室,长春,130033 |
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| 基金项目： | 吉林省科技厅重点项目"白僵菌新剂型轻型防治玉米螟配套技术研究"，中青年科技领军人才及优秀创新团队项目"吉林省农业微生物研究与利用研究创新团队" |
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| 摘 要： | 为获得理想的白僵菌分离培养基,以PPDA培养基作为基础培养基,通过观察白僵菌和杂菌在不同抑菌剂和不同抑菌剂浓度的菌落数,筛选出白僵菌生长良好,杂菌被尽可能抑制的培养基配比.结果表明,在PPDA培养基中添加70 mg/L的多果定,125 mg/L头孢霉素和138 mg/L硫酸卡那霉素为最适筛选培养基.用筛选出的培养基检测白僵菌悬乳剂和颗粒剂在田间施药后30天内的含菌量变化,发现在空气中孢子含量,白僵菌悬乳剂比颗粒剂含量更持久,证明本研究获得的筛选培养基具有实际的应用价值.
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| 关 键 词： | 球孢白僵菌 选择分离筛选培养基 菌落数 |
| 收稿时间： | 2012/12/5 0:00:00 |
| 修稿时间： | 2013/1/15 0:00:00 |
The Application and Screening of Isolation Medium for Beauveria bassiana |
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| Wang Taoyuan
,
Xu Wenjing
,
Zhang Zhengkun
,
Wang Yisheng
,
Zhao Yu
,
Wang Jingang
,
Li Qiyun.The Application and Screening of Isolation Medium for Beauveria bassiana[J].Chinese Agricultural Science Bulletin,2013,29(9):134-138. |
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| Authors: | Wang Taoyuan Xu Wenjing Zhang Zhengkun Wang Yisheng Zhao Yu Wang Jingang Li Qiyun |
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| Abstract: | In order to obtain a proper isolation medium for B. bassiana, the effects of 3 bacteriostatic agents on B. bassiana and other bacterials were determined, based on PPDA medium, and a optimized concentration of bacteriostatic agents was identified. The result showed that the PPDA medium with 70mg/L dodine, 125mg/L cephalothin and 138mg/L kanamycin sulfate is the best. The amount of B.bassiana 30 days after application of two formulation B.bassiana pesticide was detected by this new isolation medium, the data showed that the Suspoemulsion of B.bassiana were more persistence than granules in field significantly. It was proved that this new selective isolation medium was suitable to application. |
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| Keywords: | Beauveria bassiana selective isolation medium colony-forming units |
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